rexresearch.com
Martina ABRIGO, et al
Antibacterial Nanofibers
Smart dressings speed healing of chronic wounds
Researchers at Swinburne University of Technology are developing
innovative nanofibre meshes that might draw bacteria out of wounds
and speed up the healing process.
The research is the focus of Swinburne PhD candidate Martina
Abrigo, who received the university’s Chancellor's Research
Scholarship to undertake this work.
Using a technique called electrospinning – in which polymer
filaments 100 times thinner than a human hair are squeezed out of
an electrified nozzle – Ms Abrigo and her colleagues have made
nanofibre meshes that can draw bacteria from a wound.
In the first phase of research polymer nanofibres were placed over
the top of films of Staphylococcus aureus, a bacterium involved in
chronic wound infection. The researchers found the bacteria
quickly attached to the fibres.
When the fibres were smaller than the individual bacteria, fewer
cells attached and those that did attach died as they attempted to
wrap around the fibre.
In the second phase, the tiny nanofibres were coated with
different compounds and tested on the bacteria Escherichia coli,
also commonly found in chronic wounds.
The researchers found these bacteria rapidly transferred onto
fibres coated with allylamine, independent of the fibre size, but
did not attach to fibres coated with acrylic acid.
In the third phase of research, the nanofibre meshes have been
tested on tissue-engineered skin models in a partnership with
researchers at the University of Sheffield in the UK. The results
of this research are yet to be published, but indicate that
similar effects could be seen in living tissue.
“For most people, wounds heal quickly. But for some people, the
repair process gets stuck and so wounds take much longer to heal.
This makes them vulnerable to infection,” Mas Abrigo said.
“We hope this work will lead to smart wound dressings that could
prevent infections. Doctors could put a nanomesh dressing on a
wound and simply peel it off to get rid of the germs.”
[Now add silver ... ]
http://pubs.acs.org/doi/abs/10.1021/acsami.5b00453
http://scitation.aip.org/content/avs/journal/bip/10/4/10.1116/1.4927218?TRACK=RSS
Bacterial response to different surface
chemistries fabricated by plasma polymerization on electrospun
nanofibers
Martina Abrigo1, Peter Kingshott1 and Sally L. McArthur
Control over bacterial attachment and proliferation onto
nanofibrous materials constitutes a major challenge for a variety
of applications, including filtration membranes, protective
clothing, wound dressings, and tissue engineering scaffolds. To
develop effective devices, the interactions that occur between
bacteria and nanofibers with different morphological and
physicochemical properties need to be investigated. This paper
explores the influence of fiber surface chemistry on bacterial
behavior. Different chemical functionalities were generated on the
surface of electrospun polystyrene nanofibers through plasma
polymerization of four monomers (acrylic acid, allylamine,
1,7-octadiene, and 1,8-cineole). The interactions of Escherichia
coli with the surface modified fibers were investigated through a
combination of scanning electron microscopy and confocal laser
scanning microscopy. Fiber wettability, surface charge, and
chemistry were found to affect the ability of bacterial cells to
attach and proliferate throughout the nanofiber meshes. The
highest proportion of viable cells attachment occurred on the
hydrophilic amine rich coating, followed by the hydrophobic
octadiene. The acrylic acid coating rich in carboxyl groups showed
a significantly lower attraction of bacterial cells. The
1,8-cineole retained the antibacterial activity of the monomer,
resulting with a high proportion of dead isolated cells attached
onto the fibers. Results showed that the surface chemistry
properties of nanofibrous membranes can be strategically tuned to
control bacterial behavior.
KR101336251
METHOD FOR FABRICATION OF ELECTROSPUN POLYSTYRENE
NANOFIBER AND METHOD FOR FABRICATION OF ELECTROSPUN
POLYSTYRENE-QUANTUM DOT HYBRID NANOFIBER USING STYRENE MONOMER
Inventor: LEE TAE WOO, et al.
Applicant: POSTECH ACAD IND FOUND
The present invention relates to a method for manufacturing
electrospun polystyrene nanofibers and a method for manufacturing
electrospun polystyrene-quantum dot hybrid nanofibers using a
styrene monomer, without using a solvent, wherein the method for
manufacturing electrospun polystyrene-quantum dot hybrid
nanofibers comprises the steps of: manufactuirng a
polystyrene-quantum dot composite by heating the mixture of liquid
styrene monomer and a quantum dot; and a electrospinning the
polystyrene-quantum dot composite.