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PINE POLLEN








[Excerpts]

https://examine.com/supplements/pine-pollen/
Pine Pollen

Pine Pollen refers to the pollen of trees in the pinus genera, which are sometimes used as dietary supplements. Scots Pine (Pinus sylvestris) contains testosterone at levels unlikely to affect the body, while other species may have antiinflammatory properties based on preliminary evidence.

Primary Information, Benefits, Effects, and Important Facts

Pine pollen is a term used to refer to supplements derived from the pollen of pine trees. Pine trees in general refer to the genera of pinus, and the pollen that is commonly used as a dietary supplement is the Scots Pine (Pinus sylvestris) due to some studies having detected a testosterone content in this pollen.

In regards to the above, the testosterone content of Scots pine pollen appears to be too low to cause appreciable effects in the human body due to testosterone ingestion despite it being a higher concentration than the testosterone found in Royal Jelly. No studies have been conducted in humans on any parameter related to testosterone such as aphrodisia, muscle growth, or general male vitality.

Pine pollen appears to have a traditional usage in Chinese medicine as well, although the species used have been those available in the region and these are not the Scots pine. These studies are preliminary but suggest a possible antiinflammatory effect that could benefit arthritis, but due to a lack of compositional studies on the pine pollen (ie. what is actually in the pollen that could be mediating the antiinflammatory effects) it is not known if these properties extend to Scots Pine.

Overall, this supplement is heavily underresearched and at this moment in time it cannot be recommended for any particular usage in humans until more studies are conducted.



YouTube Videos

https://www.youtube.com/watch?v=Ot9Eel5PskA
How to Harvest Pine Pollen

https://www.youtube.com/watch?v=0abnh3ZP_nU
How To Harvest Pine Pollen

https://www.youtube.com/watch?v=x9pimnqbU54
Harvesting Wild Pine Pollen | Harmonic Arts

https://www.youtube.com/watch?v=OCFgdLhCIR8
How To Harvest Pine Pollen and it's Benefits

https://www.youtube.com/watch?v=ODzUp1rD_1c
Pine Pollen Harvesting, Processing and Preservation, Part 1 of 9

&c...


Our finding presents pine pollen as an attractive agent with potential to retard aging and attenuate age-related diseases in humans....

http://www.ncbi.nlm.nih.gov/pubmed/22577492
Oxid Med Cell Longev. (2012)
Antiaging effect of pine pollen in human diploid fibroblasts and in a mouse model induced by D-galactose.
Mao GX, et al.
Abstract
The present paper was designed to investigate the effect of pine pollen against aging in human diploid fibroblast 2BS cells and in an accelerated aging model, which was established by subcutaneous injections with D-galactose daily for 8 weeks in C57BL/6J mice. Pine pollen (1 mg/mL and 2 mg/mL) is proved to delay the replicative senescence of 2BS cells as evidenced by enhanced cell proliferation, decreased SA-β-Gal activity, and reversed expression of senescence-associated molecular markers, such as p53, p21(Waf1), p16(INK4a), PTEN, and p27(Kip1) in late PD cells. Besides, pine pollen reversed D-galactose-induced aging effects in neural activity and inflammatory cytokine levels, as indicated by improved memory latency time and reduced error rate in step-down test and decreased concentrations of IL-6 and TNF-α in model mice. Similar to the role of AGEs (advanced glycation endproducts) formation inhibitor aminoguanidine (AG), pine pollen inhibited D-galactose-induced increment of AGEs levels thus reversed the aging phenotypes in model mice. Furthermore, the declined antioxidant activity was obviously reversed upon pine pollen treatment, which may account for its inhibitory effect on nonenzymatic glycation (NEG) in vivo. Our finding presents pine pollen as an attractive agent with potential to retard aging and attenuate age-related diseases in humans.



Pine Elixir in Chinese Alchemy

The crane, tortoise, peach and pine tree are all symbols of longevity...

...wild, uncultivated foods of the Daoist adepts such as pine resin, needles and nuts...

Wo Quan gathered herbs and his only food were pine-seeds only. He had long hairs all over his body and he moved himself with the speed of a galloping horse (Shoushenji,1. 1b)...

Buddhist monks abstained from cereals, ate only mineral vegetable substances, wild fruits, mushrooms, pine seeds, resin needles (Mollier 2008). ...
The Japanese have a specific practice, “tree-eating” or mokujiki, to help give up the cereals which consists of eating berries, bark or pine needles....
 
The diet of Immortals, as described in such texts as Liexan zhuan and Shenxian zhuan include: “pine seeds, pine sap, pine needles, mica, sesame seeds, peach and plum blossoms, stalactite, lychee fruit, deer bamboo (huanghing), “stone grease” (a type of clay), mercury, deerhorn, chestnuts, cypress resin, sulphur, lead, the zhu plant (atractylodes macrocephala), rush and scallion roots, rape-turnip seeds, mallow (malva verticillata), turtle brains, limonite (“Yu’s leftover food”), cinnabar, bramble roots (rubus tephrodes), cantaloupe, autumn root (aconitum carmichaeli) seeds, of the zhi plant (iris florentina), the changpu plant (acorus gramineus), cinnamon, broom plant (kochia scoparia), “pine seeds that grow as parasites on mulberry trees,” niter, nions and scallions, the badou  plant (croton tiglium), realgar, sap of the arbor vitae tree (biota orientalis), flowers of the shigui tree (rhaphiolus indica), and “red flower pills” (unidentified) (Eskildsen 1998).” ...

 "During the reign of Emperor Cheng of the Han, hunters in the Zhongnan Mountains saw a person who wore no clothes, his body covered with black hair. Upon seeing this person, the hunters wanted to pursue and capture him, but the person leapt over gullies and valleys as if in flight, and so could not be overtaken. [But after being surrounded and captured, it was discovered this person was a 200 plus year old woman, who had once been a concubine of Qin Emperor Ziying. When he had surrendered to the 'invaders of the east', she fled into the mountains where she learned to subside on 'the resin and nuts of pines' from an old man. Afterwards, this diet 'enabled [her] to feel neither hunger nor thirst; in winter [she] was not cold, in summer [she] was not hot.']...



https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694268/
Int J Clin Exp Med. 2015; 8(10): 17773–17780.
PMCID: PMC4694268
PMID: 26770368
The protective effects of Masson pine pollen aqueous extract on CCl4-induced oxidative damage of human hepatic cells
Xueyuan Jin, et al.
Abstract
Objective: We observed the effects of Masson pine pollen aqueous extracts (MPPAE) on CCl4-induced oxidative damage of the human hepatic cell line L-02. Methods: We created an in vitro model of oxidative liver damage by treating L-02 human hepatic cells with 40 mmol/L CCl4. Effects of different concentrations of MPPAE on cell proliferation, morphology, and change of functional indexes were observed after addition of CCl4. Results: CCl4 was toxic to proliferation, cell morphology, and functionality of hepatic cells. It decreased proliferation by 29.3-38.4% and increased AST and ALT activities by 22.3% and 99.2%, respectively. The oxidative stress also disrupted hepatic cell growth and induced pyknosis. Although MPPAE did not prevent decreased proliferation of L-02 cells, the treatment alleviated some CCl4-induced cell morphology changes and inhibited the abnormal rise of ALT (39.8%-70.1%) and AST (14.75-27.25%) activities in a dose dependent manner. A high dose of MPPAE (400 mg/L) ameliorated nucleus deformation to an almost normal appearance. Conclusions: According to our in vitro model, MPPAE specifically prevented the changes in cell morphology and functional injury caused by CCL4 treatment; however, it offered limited protection against damage-induced reduction of proliferation.



https://www.ncbi.nlm.nih.gov/pubmed/24425185
Planta. 1976 Jan;133(1):95-6.
doi: 10.1007/BF00386012.

Protein kinase in pine pollen.
Giannattasio M1, Tucci GF, Carratù G, Carafa AM.
Abstract
Protein kinase (E.C. 2.7.1.37) activity towards both endogenous and exogenous substrates has been detected in pine pollen (Pinus canariensis P. Sm.) and partially purified by DEAE-cellulose chromatography. It seems to be not affected by cyclic AMP. Objective: We observed the effects of Masson pine pollen aqueous extracts (MPPAE) on CCl4-induced oxidative damage of the human hepatic cell line L-02. Methods: We created an in vitro model of oxidative liver damage by treating L-02 human hepatic cells with 40 mmol/L CCl4. Effects of different concentrations of MPPAE on cell proliferation, morphology, and change of functional indexes were observed after addition of CCl4. Results: CCl4 was toxic to proliferation, cell morphology, and functionality of hepatic cells. It decreased proliferation by 29.3-38.4% and increased AST and ALT activities by 22.3% and 99.2%, respectively. The oxidative stress also disrupted hepatic cell growth and induced pyknosis. Although MPPAE did not prevent decreased proliferation of L-02 cells, the treatment alleviated some CCl4-induced cell morphology changes and inhibited the abnormal rise of ALT (39.8%-70.1%) and AST (14.75-27.25%) activities in a dose dependent manner. A high dose of MPPAE (400 mg/L) ameliorated nucleus deformation to an almost normal appearance. Conclusions: According to our in vitro model, MPPAE specifically prevented the changes in cell morphology and functional injury caused by CCL4 treatment; however, it offered limited protection against damage-induced reduction of proliferation.



These results suggest that the pine pollen might be beneficial in the treatment of chronic inflammatory disorders.

http://www.ncbi.nlm.nih.gov/pubmed/19148883
Phytother Res. (2009)
Effect of pine pollen extract on experimental chronic arthritis.
Lee KH, Choi EM.

Abstract
The effects of pine pollen extract (PE) on Freund's complete adjuvant (FCA)-induced arthritis and collagen-induced arthritis (CIA) were investigated. The oral administration of PE (100 and 200 mg/kg per day) for 21 days after subcutaneous immunization with FCA, significantly reduced hindpaw swelling and the production of inflammatory cytokines (TNF-alpha, IL-1beta and IL-6) compared with the FCA-induced arthritis group. Treatment with the PE (100 mg/kg) also decreased the serum levels of LDL-cholesterol and increased HDL-cholesterol contents compared with those of the arthritis group. Since CIA is a model of some types of human autoimmune rheumatoid arthritis (RA), the study examined whether PE is efficacious against CIA in mice and investigated the possible antioxidant potential of PE on CIA. Arthritis in DBA/1J mice was induced by subcutaneous immunization with bovine type II collagen. PE (100 and 200 mg/kg) was orally administered once daily for 49 days after initial immunization with type II collagen. The arthritis score and paw edema were markedly suppressed in the groups treated with PE. Moreover, administration of PE (100 mg/kg) for 49 days reduced the serum levels of rheumatoid factor, anti-type II collagen antibody, TNF-alpha, IL-1beta, IL-6, protein carbonyl, advanced glycation endproducts, malondialdehyde and LDL-cholesterol compared with that of CIA mice. These results suggest that the pine pollen might be beneficial in the treatment of chronic inflammatory disorders.



http://onlinelibrary.wiley.com/doi/10.1002/ptr.2103/abstract
https://doi.org/10.1002/ptr.2103
Antinociceptive and antiinflammatory activities of pine (Pinus densiflora) pollen extract.
Eun‐Mi Choi
Abstract
The study aimed to evaluate the antinociceptive and antiinflammatory activity of pine (Pinus densiflora) pollen in mice. The antinociceptive activity was determined using acetic acid‐induced abdominal constriction and formalin‐induced licking, and the hot plate test. Antiinflammatory effects were evaluated using carrageenan‐ and formalin‐induced paw edema, and arachidonic acid‐induced ear edema in mice. The ethanol extract of pine pollen (100 and 200 mg/kg, p.o.) produced a significant inhibition of both phases of the formalin pain test in mice, a reduction in mouse writhing induced by acetic acid and an elevation of the pain threshold in the hot plate test in mice. The pine pollen extract also produced a significant inhibition of carrageenan‐ and formalin‐induced paw edema as well as arachidonic acid‐induced ear edema in mice. The inhibitions were similar to those produced by aminopyrine and indomethacin, p.o. The different polyphenols found in pine pollen could account for the antinociceptive and antiinflammatory actions. The results obtained indicate that the extract possesses analgesic and antiinflammatory effects.   



http://www.ncbi.nlm.nih.gov/pubmed/16499047
Analysis of pine pollen by using FTIR, SEM and energy-dispersive X-ray analysis.
Guang Pu Xue Yu Guang Pu Fen Xi. (2005), Wang YM, Wang HJ, Zhang ZY.
Abstract
The study aimed to evaluate the antinociceptive and antiinflammatory activity of pine (Pinus densiflora) pollen in mice. The antinociceptive activity was determined using acetic acid‐induced abdominal constriction and formalin‐induced licking, and the hot plate test. Antiinflammatory effects were evaluated using carrageenan‐ and formalin‐induced paw edema, and arachidonic acid‐induced ear edema in mice. The ethanol extract of pine pollen (100 and 200 mg/kg, p.o.) produced a significant inhibition of both phases of the formalin pain test in mice, a reduction in mouse writhing induced by acetic acid and an elevation of the pain threshold in the hot plate test in mice. The pine pollen extract also produced a significant inhibition of carrageenan‐ and formalin‐induced paw edema as well as arachidonic acid‐induced ear edema in mice. The inhibitions were similar to those produced by aminopyrine and indomethacin, p.o. The different polyphenols found in pine pollen could account for the antinociceptive and antiinflammatory actions. The results obtained indicate that the extract possesses analgesic and antiinflammatory effects.



http://www.ncbi.nlm.nih.gov/pubmed/3671475
Pharmazie. (1987)
Vitamin D and its metabolites in the pollen of pine. Part 5: Steroid hormones in the pollen of pine species.
Saden-Krehula M, Tajíc M.
Abstract
Unconjugated vitamin D and its metabolites were investigated in the pollen of Pinus nigra Ar. and Pinus sylvestris L. by TLC, HPLC and competitive radiochemical determination of 25-hydroxycholecalciferol (25-OHD3). It was found that vitamin D (D2, D3) was present in the pollen in amounts about 2 micrograms/10 g and 25-OHD3, 24,25-dihydroxycholecalciferol [24,25-(OH)2D3] and 1,25-dihydroxycholecalciferol [1,25-(OH)2D3] between 0.1 and 3 micrograms/10 g of pollen, dependent on pollen species and methods.



https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4341283/
Pine pollen inhibits cell apoptosis-related protein expression in the cerebral cortex of mice with arsenic poisoning
Abstract
Previous studies have demonstrated that pine pollen can inhibit cerebral cortical cell apoptosis in mice with arsenic poisoning. The present study sought to detect the influence of pine pollen on apoptosis-related proteins. Immunohistochemistry, western blotting and enzyme-linked immunosorbent assays were used to measure the levels of apoptosis-related proteins in the cerebral cortex of mice with arsenic poisoning. Results indicated that pine pollen suppressed cell apoptosis in the cerebral cortex of arsenic-poisoned mice by reducing Bax, Bcl-2 protein expression and increasing p53 protein expression.



http://www.ncbi.nlm.nih.gov/pubmed/19107823
Phytother Res. 2009 Jan;23(1):41-8. doi: 10.1002/ptr.2525.
Antioxidant and antiinflammatory activity of pine pollen extract in vitro.
Lee KH, Kim AJ, Choi EM
Abstract
To determine the medicinal properties of pine pollen, the antioxidant and antiinflammatory activities of the ethanol extract of pine pollen extract (PPE) were investigated. PPE displayed a strong free radical scavenger activity on 1,1-diphenyl-2-picrylhydrazyl radical and hydrogen peroxide. It was observed also that the antioxidant activity, measured by the ferric thiocyanate method, increased with the addition of PPE to the linoleic acid emulsion system. PPE was also found to inhibit significantly the amount of malondialdehyde and protein carbonyls formed from liver homogenate. Like the antioxidant activity, the reducing power of PPE was excellent. Thereafter, the study investigated the effects of PPE in modulating the production of pro-inflammatory mediators in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages, and the effect of PPE on interleukin (IL)-1beta-induced matrix metalloproteinases (MMPs) production and mitogen-activated protein kinases (MAPKs) activation in the human synovial sarcoma cell line, SW982. PPE was found to inhibit the production of nitric oxide, tumor necrosis factor-alpha, IL-1 and IL-6 in LPS-activated macrophages. Treatment with PPE at 10 microg/mL significantly (p < 0.05) inhibited IL-1beta-induced MMPs (MMP-1 and -3) production in SW982 cells. IL-1beta-induced JNK activation was inhibited by PPE (10 microg/mL), whereas p38 and ERK1/2 were not affected. These findings suggest that pine pollen is a potential antioxidant and beneficial for inflammatory conditions through down-regulation of JNK and MMPs.



http://www.ncbi.nlm.nih.gov/pubmed/5549221
Experientia. 1971 Jan 15;27(1):108-9.
Testosterone, epitestosterone and androstenedione in the pollen of Scotch pine P. silvestris L.
Saden-Krehula M, Tajic M, Kolbah D.



https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1916459/
Am J Pathol. 1966 Jul; 49(1): 153–165. / PMCID: PMC1916459 / PMID: 5944757

Experimental pine pollen granulomatous pneumonia in the rat.
D. O. Adams



https://www.ncbi.nlm.nih.gov/pubmed/13571238
Am J Med Sci. 1958 Sep;236(3):311-7. / PMID:   13571238
Chemical constituents of pine pollen and their possible relationship to sarcoidosis.
CUMMINGS MM, HUDGINS PC.



Patents for Pine Pollen Extraction
http://www.espacenet.com

CN1307092

Supercritical CO2 extractor, pine pollen
In a supercritical CO2 extractor, pine pollen after drying, wall braking, and disinfection is extracted with CO2 for 0.5-2 hr at the condition of 30-50 deg.c temperature, 8-40 MPa pressure and 0.5-15L/min CO2 flow rate. In a separator, fat material dissolved in CO2 fluid is separated and CO2 enters the extractor for circular extraction. Treated pine pollen may be used in medicine, food and beautifying skin-care product and the extracted fat material has high quality, low cost and no organic solvent contained.

CN104983753
Extracting technology and a measuring method of pine pollen total saponins
The invention discloses an extracting technology and a measuring method of pine pollen total saponins. The extracting technology is characterized by comprising the steps that dried pine pollen with broken walls is taken, petroleum ether is degreased, drying is carried out, and then degreased pine pollen is obtained; the degreased pine pollen is extracted repeatedly with ethyl alcohol two times; filtrates are combined, and vacuum concentration is carried out until alcohol taste is removed, after distilled water is used for dissolving, and n-butyl alcohol is used for repeated extraction until the color is removed; and the n-butyl alcohol is combined, vacuum concentration is carried out, frozen drying is carried out, and then the pine pollen total saponin product is obtained. The measurement of the content of the pine pollen total saponins is characterized in that ginsenoside RE is taken as a standard product, and ultraviolet spectrophotometry is used for detecting the content of the pine pollen total saponins; the technology is adopted for extracting the pine pollen total saponins, and is simple, high in operability and good in extraction effect; and the extraction rate can account for 0.93% of raw materials by weight, the measuring method is simple, reliable, easy to operate, high in precision and good in repeatability.

CN104189024
Extraction method for pin pollen active substances.
The invention relates to an extraction method for pin pollen active substances. The method comprises the following steps of (1) sorting the pine pollen by vibration with a 200-mesh vibrating screen; (2) carrying out deep impurity separation on the screened pine pollen raw powder by using an airflow classifier to remove dust and fine impurities from the pine pollen raw powder; (3) breaking the pine pollen raw powder to below 2-3 micrometers by a low-temperature physical wall-breaking technology; (4) granulating the broken pine pollen with a proper amount of an adhesive; (5) injecting pretreated pine pollen and super-critical CO2 into an extraction kettle; and (6) separating the active substances of the pine pollen by reducing pressure and raising a temperature to obtain the grease of the active substances. The extraction method is beneficial for release and extraction of pine pollen nutritional ingredients and active substances; a comprehensive extraction rate of the active substances of the pine pollen can reach over 98%, 4%-8% higher than that of other extraction methods; and occurrence of residual organic solvents can be prevented.

CN103130902
Method for extracting polysaccharide from pine pollen.
The invention relates to a method for extracting polysaccharide from pine pollen. The method for extracting the polysaccharide from the pine pollen is characterized by comprising the following steps. Firstly, wall-breaking treatment is performed on the pine pollen, and the pine pollen is obtained. Secondly, the treatment of ultrasound and hot water extraction is performed on wall-breaking pollen, stirring is performed, the treatment of centrifugation is performed, filtering is performed and then supernate is obtained. Thirdly, the supernate is processed by an ultrafiltration membrane filtration system, and collected fluid is collected. Fourthly, trichloroacetic acid is added in the collected liquid, the treatment of centrifugation is performed, fibronectin in the collected liquid is removed, and a polysaccharide solution is obtained.; Fifthly, vacuum concentration is performed on the polysaccharide solution, and a polysaccharide concentrated solution is obtained. Sixthly, the treatment of vacuum freeze drying is performed on the polysaccharide concentrated solution, and the polysaccharide is obtained. The method for extracting the polysaccharide from the pine pollen is simple in technology, strong in operability, capable of saving a large quantity of organic solvents, explosive compounds of ethanol, chloroform, n-butyl alcohol and the like are not needed, and capable of reducing the risks in production of products.

CN102940704
Pine pollen water extract buccal tablet for preventing and treating alcoholic fatty liver   
The invention relates to a pine pollen water extract buccal tablet for preventing and treating alcoholic fatty liver, relating to the technical field of a process for extracting effective substances from plants, particularly from pine pollen. The pine pollen water extract buccal tablet mainly comprises freeze-dried powder of pine pollen water extracts, folia perillae acutae powder, hawthorn fruit powder, isomaltitol and magnesium stearate, wherein the pine pollen water extracts are prepared through a bee pollen water extraction method and have molecular weight being less than 5000. The pine pollen water extract buccal tablet disclosed by the invention has an obvious effect of preventing and controlling the alcoholic fatty liver without side effect and is low in cost and simple and easy to prepare.

CN102488043
Anti-fatigue life-prolonging pine tea, particle containing the anti-fatigue life-prolonging pine tea and pine capsule containing the particle
The invention discloses anti-fatigue life-prolonging pine tea, a particle containing the anti-fatigue life-prolonging pine tea and a pine capsule containing the particle. A preparation method of the anti-fatigue life-prolonging pine tea comprises the following steps of mixing pine needle, pine bark, cypress leaf, cypress bark, mulberry leaf, lophatherum gracile, fleece flower root, wolfberry, Chinese yew, ginkgo leaf, root of pilose asiabell, eucommia ulmoides, poria cocos, manyflower solomonseal rhizome, raisin and mix-fired licorice, adding water into the mixture, wherein the volume of the added water is 8 time the volume of the mixture, carrying out a decoction extraction process for 2 hours, adding water into the decoction extraction products, wherein the volume of the added water is 6 time the volume of the decoction extraction products, carrying out a decoction extraction process for 2 hours, merging the extract obtained by the decoction extraction processes, carrying out condensation and drying to obtain dry paste, merging the dry paste, crushing the merged dry paste into fine powder, adding pine pollen, bee pollen, milk powder, cane sugar, dextrin and edible essence into the fine powder, mixing, and packaging the mixture by bag to obtain the anti-fatigue life-prolonging pine tea. The particle containing the anti-fatigue life-prolonging pine tea is prepared from the anti-fatigue life-prolonging pine tea and an auxiliary material of starch. The particles containing the anti-fatigue life-prolonging pine tea are put into a hollow capsule so that the pine capsule is obtained. The anti-fatigue life-prolonging pine tea, the particle containing the anti-fatigue life-prolonging pine tea and the pine capsule containing the particle have the advantages of obvious effects, simple taking method and no toxic and side effect.

CN102229973
Extraction method of Chinese pine pollen small peptides
The invention relates to an extraction method of Chinese pine pollen small peptides. The extraction method comprises the following steps of (1) mixing Chinese pine pollen and water, carrying out a wall breaking process on the Chinese pine pollen in a wet way through a colloid mill, and filtering, (2) extracting a soluble component from the Chinese pine pollen treated by the wall breaking process through adopting a solvent oil, (3) putting the Chinese pine pollen treated by the step 3 into a container with a stirrer, adding filtrate obtained from the step 1 into the Chinese pine pollen, adjusting a pH of the mixture to a pH of 8 to 9, and stirring the mixture for 2 to 4 hours at a temperature of 20 to 70 DEG C to obtain Chinese pine pollen mixed solution, and (4) carrying out processes of enzymatic hydrolysis and enzyme destruction, carrying out a centrifugal separation process,; and removing residues to obtain nutrient solution rich in small peptides. The extraction method has a short production period and a high product yield. Nutrient components of extract produced by the extraction method are micro-molecule peptides which can be absorbed by human bodies easily. Active micro-molecule peptide nutrient solution prepared by the extraction method has a high water-solubility and can be used directly.

CN101857628
Method for extracting and separating phytosterol from pine pollen
The invention relates to a method for extracting and separating phytosterol from pine pollen, which comprises the following steps of: (1) performing pretreatment on the pine pollen to obtain the pine pollen for supercritical CO2 extraction; (2) extracting the phytosterol from the pine pollen by using the supercritical CO2 extraction, namely allowing the CO2 to enter a high pressure pump after the CO2 is condensed in an ice chest to become liquid, allowing the pressurized CO2 to enter an extractor in an extraction device after the CO2 is heated by a heater, making the CO2 fully contacted with the pine pollen in the extractor, and then performing continuous extraction on the pine pollen under the conditions that the pressure of an extraction kettle is 35 to 50MPa, the extracting temperature is between 50 and 70 DEG C, the flow rate of the CO2 is 20 to 30L/h, and the extracting time is 0.5 to 2 hours; and (3) enriching the phytosterol in the extract. The method has the advantages that: (1) deep comprehensive development of the pine pollen is realized; and (2) the quality of the extract of the pine pollen is high, and the phytosterol content is high.

CN101756088
Preparation method of novel pine pollen preparation
The invention discloses a preparation method of a novel pine pollen preparation, which comprises the following steps of: using pine pollen as a raw material without using any additive, simulating the environment suffered by a medicament in the transportation process of the digestive tract of a human body after ultramicro wall breaking, keeping a lower temperature, carrying out effective component extraction for 60 percent of the gross of the wall-broken pine pollen for many times under the conditions of pH 2, pH 7.6 and pH 8.4, and then mixing an extract with the rest 40 percent of the wall-broken pine pollen to prepare a super-concentrated pine pollen capsule or tablet. Because concentration extraction is carried out for most of the raw material through a biomimetic process at the low temperature by the method, the utilization ratio of natural resources is greatly improved, so that a product has high and abundant nutrient contents and simultaneously is more easily digested and absorbed by the human body, and meanwhile, the preparation formulation is greatly shrunk, so the novel pine pollen preparation is easy to take and convenient to carry.

CN101385746
Extraction method of effective ingredient from pine pollen using supercritical carbon dioxide extraction
The invention provides a method for extracting active ingredients of pollen pini by supercritical CO2, including the following steps: a. loading pretreated pollen pini into an extracting kettle; b. injecting supercritical CO2 into the extracting kettle, extracting with the CO2 flow speed of 8 to 18 l/min at the temperature of 35 to 50 DEG C and under the pressure of 30 to 40 MPa; c. CO2 fluid dissolved with active ingredients entering a separation kettle for separation. The method has simple technology and convenient operation, can avoid remain of organic solvents, greatly enhances production efficiency and raises extracting contents of phospholipids and total phytosterols.

CN101099752
Method for extracting pollen essential from Chinese pine pollen
The present invention relates to a method for extracting pollen essence from Chinese pine pollen. Said extraction method includes the following several steps: making Chinese pine pollen undergo the processes of wall-breaking and drying treatment; adopting ethyl alcohol solution whose concentration is 5-95% as solvent, fully mixing ethyl alcohol solution and Chinese pine pollen according to the liquid-solid ratio of 20-5:1, soaking for 2-5h at 20-70deg.C to make primary extraction; filtering to obtain primary filter residue and primary filtrate; making the primary filtrate undergo the processes of pressure reduction, distillation and concentration so as to obtain primary pollen essence concentrate; then making the primary pollen essence concentrate undergo the process of drying the solvent by utilizing distillation at low temperature so as to obtain the invented primary pollen essence.





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