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FUCOIDAN EXTRACTION
https://en.wikipedia.org/wiki/Fucoidan
Fucoidan
Fucoidan is a sulfated polysaccharide (MW: average 20,000) found mainly in various species of brown algae and brown seaweed such as mozuku, kombu, bladderwrack, wakame, and hijiki (variant forms of fucoidan have also been found in animal species, including the sea cucumber). Fucoidan is used as an ingredient in some dietary supplement products.
Fucoidan designates a group of certain fucose-containing sulfated polysaccharides (FCSPs) that have a backbone built of (1?3)-linked a-l-fucopyranosyl or of alternating (1?3)- and (1?4)-linked a-l-fucopyranosyl residues, and also include sulfated galactofucans with backbones built of (1 ? 6)-ß-d-galacto- and/or (1 ? 2)-ß-d-mannopyranosyl units with fucose or fuco-oligosaccharide branching, and/or glucuronic acid, xylose, or glucose substitutions.[1][2] These FCSPs offer several potentially beneficial bioactive functions for humans. The bioactive properties may vary depending on the source of seaweed, the compositional and structural traits, the content (charge density), distribution, and bonding of the sulfate substitutions, and the purity of the FCSP product. The preservation of the structural integrity of the FCSP molecules essentially depends on the extraction methodology which has a crucial, but partly overlooked, significance for obtaining the relevant structural features required for specific biological activities and for elucidating structure-function relations.[1][3]
Research
Fucoidins have been the subject of preliminary studies to investigate claims for potential antioxidant, cognitive, anti-inflammatory, anti-angiogenic, anti-cancer, anti-viral, and anti-hyperglycemic effects.[4]
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413214/
doi: 10.3390/md13042327
Mar Drugs. 2015 Apr; 13(4): 2327–2346.
Fucoidan and Cancer: A Multifunctional Molecule with Anti-Tumor PotentialAbstract
Farzaneh Atashrazm, et al.
There is a wide variety of cancer types yet, all share some common cellular and molecular behaviors. Most of the chemotherapeutic agents used in cancer treatment are designed to target common deregulated mechanisms within cancer cells. Many healthy tissues are also affected by the cytotoxic effects of these chemical agents. Fucoidan, a natural component of brown seaweed, has anti-cancer activity against various cancer types by targeting key apoptotic molecules. It also has beneficial effects as it can protect against toxicity associated with chemotherapeutic agents and radiation. Thus the synergistic effect of fucoidan with current anti-cancer agents is of considerable interest. This review discusses the mechanisms by which fucoidan retards tumor development, eradicates tumor cells and synergizes with anti-cancer chemotherapeutic agents. Challenges to the development of fucoidan as an anti-cancer agent will also be discussed.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117709/
Mar Drugs. 2018 Aug; 16(8): 257.
doi: 10.3390/md16080257
Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted ExtractionAbstract
Marco Garcia-Vaquero, et al.
The objectives of this study were to employ response surface methodology (RSM) to investigate and optimize the effect of ultrasound-assisted extraction (UAE) variables, temperature, time and amplitude on the yields of polysaccharides (fucose and total glucans) and antioxidant activities (ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity (DPPH)) from Laminaria digitata, and to explore the suitability of applying the optimum UAE conditions for L. digitata to other brown macroalgae (L. hyperborea and Ascophyllum nodosum). The RSM with three-factor, four-level Box-Behnken Design (BBD) was used to study and optimize the extraction variables. A second order polynomial model fitted well to the experimental data with R2 values of 0.79, 0.66, 0.64, 0.73 for fucose, total glucans, FRAP and DPPH, respectively. The UAE parameters studied had a significant influence on the levels of fucose, FRAP and DPPH. The optimised UAE conditions (temperature = 76 °C, time = 10 min and amplitude = 100%) achieved yields of fucose (1060.7 ± 70.6 mg/100 g dried seaweed (ds)), total glucans (968.6 ± 13.3 mg/100 g ds), FRAP (8.7 ± 0.5 µM trolox/mg freeze-dried extract (fde)) and DPPH (11.0 ± 0.2%) in L. digitata. Polysaccharide rich extracts were also attained from L. hyperborea and A. nodosum with variable results when utilizing the optimum UAE conditions for L. digitata.
1. Introduction
Macroalgae are a diverse group of organisms capable of adapting to the extreme marine environmental conditions by producing multiple bioactive compounds. Marine macroalgae are considered a rich source of micro- and macronutrients with antioxidant activities, i.e., minerals, carotenoids, phenolic compounds, proteins and polysaccharides [1].
Macroalgal polysaccharides, particularly fucoidan and laminarin have a wide range of biological activities such as antioxidant, immunostimulatory and anti-microbial both in vitro and in vivo [2,3]. Fucoidans are a family of sulphated fucose-rich polysaccharides, built on a backbone of a-l-fucopyranose residues [4]. These polysaccharides are an integral part of the cell walls of brown macroalgae, playing a crucial role in the protection of seaweeds against environmental challenges [5]. Laminarins are glucan-polysaccharides containing 1,3-linked ß-d-glucose residues with different degrees of 6-O branching and ß-(1,6) intrachain links [6]. Hence, glucans are polysaccharides of d-glucose monomers that act as energy reserve compounds stored in vacuoles inside the macroalgal cells [7].
The traditional extraction methods employed for polysaccharides involve the use of one or several solvents used alone or in combination with high temperatures [2]. Innovative technologies are currently being explored to generate novel extraction protocols, aiming to obtain higher yields of bioactive compounds and to develop more environmentally friendly processes, with lower energy consumption, time and cost of extraction [8]. Ultrasound-assisted extraction (UAE) is one of the most promising innovative technologies used to date [9]. The enhancement in the extraction of bioactive compounds achieved by UAE is mainly attributed to the effect of cavitations in the solvent [10]. The process of cavitation involves nucleation, growth and collapse of bubbles in a liquid, driven by the bulk pressure variation created by the passage of the ultrasound waves. The cavitation creates physical effects, such as velocity/pressure shockwaves that causes cell disruption, and micro-turbulences that mixes and accelerates the extraction of bioactive compounds through the cell membranes [8,10]. The UAE has been utilized to extract multiple functional molecules from plants [11,12,13], macroalgae [14,15] and microalgae [16]...
2.2. Optimization of the Extraction of Polysaccharides and Antioxidant Activity
The current study focuses on the extraction of both fucose and glucans together along with their antioxidant activity by optimizing time, temperature and amplitude. All extraction parameters were optimized by using a more powerful semi-industrial ultra-sonication device (power 500 W, 20 kHz), compared to a lab grade ultra-sonication device used in previous studies [7,20]. Optimum conditions were determined aiming to maximize the yields of (i) fucose (condition 1), (ii) total glucans (condition 2), (iii) antioxidant activities (FRAP and DPPH) (condition 3) and (iv) yield of polysaccharides and antioxidant activities combined (condition 4). The levels of the three independent parameters used in UAE (temperature, time and ultrasonication amplitude), together with the predicted values and the experimental results obtained from L. digitata extracts are summarized in Table 4. The predicted values of the theoretical model for the four optimum conditions described were confirmed with the experimental data with the exception of the FRAP values, which were lower than the predicted values in both conditions 3 and 4.
The optimum UAE extraction conditions to obtain high yields of fucose from L. digitata were temperature (80 °C), time (30 min) and ultrasonication amplitude (40%; condition 1; Table 4). There is some conflicting data in the literature with regard to the influence of these conditions on the yields of fucose. Previous studies using UAE did not identify an influence of time or amplitude on the fucose content of extracts from A. nodosum [20]. Our results suggested that temperature is a critical factor for getting higher yield of both fucose and glucans along with total antioxidant activity, which was neglected in previous studies [7,20]. Our results are in agreement with Ale et al. [22] wherein the temperature and time of extraction also had an influence on the extraction of fucose from Sargassum spp. using conventional extraction techniques, with optimum extraction conditions obtained at temperatures of 90 °C over a 4 h period. However, previous researchers optimizing UAE conditions to obtain bioactive compounds from plants identified an influence of temperature, time and various ultrasonication parameters (i.e., frequency and power) on the yields of polysaccharides [23,24].
The optimum UAE extraction conditions to obtain high yields of total glucans from L. digitata were temperature (52.5 °C), time (10 min) and ultrasonication amplitude (100%; condition 2; Table 4). High ultrasonication amplitudes were also required to recover glucans from mushroom by-products (Agaricus bisporus) with the highest yields of glucans obtained applying high ultrasonic amplitudes (100 µm) for 15 min, followed by 1 h of precipitation with ethanol [25]. A previous study carried out by Kadam et al. [7] using 0.1 M HCl showed an increased extraction of glucans from L. hyperborea and A. nodosum at 60% of ultrasonic amplitude for 15 min, although the optimization of the UAE parameters was not performed [7].
The optimum UAE extraction conditions to obtain high yields of fucose from L. digitata were temperature (80 °C), time (30 min) and ultrasonication amplitude (40%; condition 1; Table 4). There is some conflicting data in the literature with regard to the influence of these conditions on the yields of fucose. Previous studies using UAE did not identify an influence of time or amplitude on the fucose content of extracts from A. nodosum [20]. Our results suggested that temperature is a critical factor for getting higher yield of both fucose and glucans along with total antioxidant activity, which was neglected in previous studies [7,20]. Our results are in agreement with Ale et al. [22] wherein the temperature and time of extraction also had an influence on the extraction of fucose from Sargassum spp. using conventional extraction techniques, with optimum extraction conditions obtained at temperatures of 90 °C over a 4 h period. However, previous researchers optimizing UAE conditions to obtain bioactive compounds from plants identified an influence of temperature, time and various ultrasonication parameters (i.e., frequency and power) on the yields of polysaccharides [23,24].
The optimum UAE extraction conditions to obtain high yields of total glucans from L. digitata were temperature (52.5 °C), time (10 min) and ultrasonication amplitude (100%; condition 2; Table 4). High ultrasonication amplitudes were also required to recover glucans from mushroom by-products (Agaricus bisporus) with the highest yields of glucans obtained applying high ultrasonic amplitudes (100 µm) for 15 min, followed by 1 h of precipitation with ethanol [25]. A previous study carried out by Kadam et al. [7] using 0.1 M HCl showed an increased extraction of glucans from L. hyperborea and A. nodosum at 60% of ultrasonic amplitude for 15 min, although the optimization of the UAE parameters was not performed [7].
The mild extraction conditions needed to preserve the antioxidant activities (FRAP and DPPH) of extracts from L. digitata (temperature 40 °C, time 30 min and amplitude 40%; condition 3; Table 4) could be due to the antioxidant power of other thermolabile compounds that could be present in the crude extracts, such as proteins/peptides [1] and polyphenols [26,27]. In fact, previous studies optimizing UAE to achieve phenolic compounds from brown macroalgae (Hormosira banksia) obtained maximum phenolic contents using low temperatures (30 °C) at medium sonication power (60%) for 60 min [28].
The optimum conditions to obtain both high yields of polysaccharides and antioxidant activities were of temperature (76 °C), time (10 min) and ultrasonication amplitude (100%; condition 4; Table 4). To our knowledge there are no studies presented in the literature that aim to optimize the yields of polysaccharides and its antioxidant activities from any species of seaweed.
2.3. Application of Optimal UAE Conditions in other Brown Macroalgae
The applicability of the four optimum conditions for L. digitata was subsequently explored to generate polysaccharide rich extracts from other brown macroalgae with commercial value (L. hyperborea and A. nodosum). The contents of fucose, total glucans and antioxidant activities (FRAP and DPPH) of extracts from L. hyperborea and A. nodosum using optimal UAE conditions are compiled in Table 5. L. hyperborea extracts had higher contents of total glucans and DPPH activities, being approximately 10 and 4 fold higher than the values obtained from L. digitata, respectively. A. nodosum extracts showed powerful antioxidant activities (FRAP and DPPH) when compared to both Laminaria species. Previous studies aiming the UAE of fucose and glucans from brown macroalgae achieved extracts containing 87.06 mg fucose/g from A. nodosum [20] and 5.29–6.24 mg glucans/100 mg from L. hyperborea and A. nodosum, although the antioxidant activity of these extracts was not reported [7]...
4. Conclusions
Ultrasound-assisted extraction (UAE) was studied for the extraction of polysaccharides (fucose and glucans) and antioxidant activities (FRAP and DPPH) from L. digitata. Response surface methodology was employed to investigate the effect of the UAE variables (temperature, time and ultrasonic amplitude) on the macroalgal extracts to enhance the yields of polysaccharides and its antioxidant activities. The UAE parameters studied showed significant influence on the levels of fucose, FRAP and DPPH. Levels of 1060.75 mg/100 g ds, 968.57 mg/100 g ds, 8.70 µM trolox/mg fde and 11.02% were obtained for fucose, total glucans, FRAP and DPPH respectively at optimized conditions of temperature (76 °C), time (10 min) and ultrasonic amplitude (100%) using 0.1 M HCl as solvent. The UAE conditions described were then applied successfully to other economically relevant brown macroalgae (L. hyperborea and A. nodosum) to obtain polysaccharide rich extracts. This study demonstrates the applicability of UAE to enhance the extraction of bioactive polysaccharides from various macroalgal species.
Fucoidan Extraction Patents
METHOD OF COMPLEX PROCESSING OF BROWN ALGAE
RU2676271
FIELD: pharmaceuticals.SUBSTANCE: invention relates to pharmaceutical industry, in particular to a method for complex processing of fucus algae. Method of complex processing of fucus algae with simultaneous production of a full range of biologically active substances from algae biomass in a single technological cycle, which consists in processing ground air-dry raw materials by supercritical fluid extraction, solvent - supercritical CO, co-solvent ethanol, the obtained supercritical extract is divided into fractions with the prevailing content of components - polyphenols and fatty acids, after the supercritical fluid extraction algal residue is subjected to extraction with water with constant stirring, the extract is evaporated on a rotary evaporator and separated by addition of ethyl alcohol, the precipitated laminaran and fucoidan are separated from the solution by centrifuging, supernatant is cooled and left to precipitate mannitol from the solution for a day, then ethanol is removed from the aqueous-alcoholic extract using a rotary evaporator, evaporating the aqueous-alcoholic extract to the original volume, the resulting aqueous solution is diluted, acidified with concentrated hydrochloric acid and three-fold liquid-phase extraction of the polyphenol fraction from an aqueous solution of brown algae with a mixture of ethyl acetate and n-butanol is carried out, then after aqueous extraction the algal residue is treated with a solution of NaHCO with hydronic module, extracts are combined, acidified with concentrated sulfuric acid, the precipitated sodium alginate is used to produce various alginic acid salts, after the separation of alginates fibrous residue - algal fiber is purified by fourfold extraction with water at the boiling point of the solvent with a water duty, under certain conditions. EFFECT: described method allows to obtain not only a carbohydrate fraction (mannitol, alginic acids, fucoidan), but also a lipid-pigment complex, the polyphenolic complex and the algal fiber from the algal biomass.
Method for extracting fucoidan sulfate from sporophyll of undaria pinnatifida and removing heavy metals
CN108641008
The invention discloses a method for extracting fucoidan sulfate from sporophyll of undaria pinnatifida and removing heavy metals, wherein the method includes the following steps: raw material pretreatment, fucosan sulfate extraction, heavy metal removal, algin removal by primary precipitation, and fucoidan sulfate production by secondary precipitation. Terahertz wave extraction is adopted, and the extraction efficiency is high. Heavy metals are treated and removed by combination of terahertz waves, ion exchange and electrodialysis, the removal range of heavy metal ions is wide and the removal rate is high. The purity of the obtained product is high, and the product can be used as health food raw materials to develop high value-added products and improves the economic value of raw materials.
Extraction and separation technology for fucoidan extract
CN108484788
The invention relates to an extraction and separation technology for a fucoidan extract and belongs to the technical field of extraction and separation. According to the technical scheme, the technology comprises the following steps of cutting and soaking, wherein dried undaria pinnatifida, dried sargassum fusiforme, dried kelp and other brown algae are taken, soaked and cut into brown algae shreds, drinking water is added, the soaking temperature is 25-60 DEG C, and stirring and extraction are conducted for 5-10 h; centrifugation and separation, wherein the mixed liquid is centrifuged and separated, impurities are filtered, and a filtrate is obtained; concentration, wherein the filtrate is concentrated by an ultra-filtration membrane; acidification, wherein a concentrated solution is stirred, hydrochloric acid is added, the pH of a material solution is adjusted and stabilized within 2-4, stirring is conducted for 30 min, and then standing is conducted for 6-10 h; filter pressing and separation, wherein the liquid is subjected to filter pressing and separation, impurities are removed, and a filtrate is obtained; concentration and washing, wherein the filtrate is concentrated, the pH is adjusted to 6-8 by using alkaline liquor, and purified water is added for washing; drying, wherein the concentrated solution is subjected to microwave drying, and a brown algae extraction solid is obtained. Compared with an alcohol precipitation method and the like, the extraction and separation technology has the advantages that the cost is greatly lowered, a microwave method is used for drying, the operation steps are greatly reduced, and the efficiency is improved.
Extraction method of fucoidan
KR101866906
The present invention relates to a method for extracting fucoidan. More specifically, the present invention relates to a method for extracting fucoidan, comprising the following steps: (a) preparing a first mixture by mixing choline chloride and ethylene glycol; (b) adding water to the first mixture and stirring the same to prepare a second mixture; (c) crushing kelp, adding a solvent, and stirring the same to remove impurities; and (d) mixing the second mixture and the kelp from which the impurities have been removed, and then heating the same to obtain fucoidan.
METHOD FOR OBTAINING POLYSACCHARIDE-CONTAINING POLYMER MATRICES
RU2657608
FIELD: chemistry.SUBSTANCE: invention relates to the chemical-pharmaceutical industry, particularly to a method for producing polysaccharide-containing polymer matrices, which comprises mixing, in a volume ratio of 2:1:2, 30 % acrylamide solution in 1 % methylene bisacrylamide solution, a tris buffer with pH=7.7 and a 1-2 % aqueous polysaccharide solution, adding the resulting solution in a dropwise manner to n-hexane, stirring at a rate of 500 rpm for 1-2 minutes, adding 10 % ammonium persulphate solution to start polymerisation, stirring for 30 minutes, adding a sorbitan monooleate emulsifier while continuing to stir for 15 minutes, then distilling off the n-hexane on a rotary evaporator, distributing the resulting matrices on filter paper, drying, holding in a refrigerator for 48 hours at a temperature of 4-8 °C, washing with chloroform, holding in a desiccator at 4 °C for 100-120 h, wherein said polysaccharide solution used is either an aqueous solution of fucoidan obtained by fractional extraction from brown alga Laminaria saccharina, or an aqueous solution of mannan obtained by separation of yeast autolysate Saccharomyces cerevisiae at a temperature of 45-50 °C for 20-24 hours, or a mixture, in volume ratio of 1:1, of said aqueous solution of fucoidan and said aqueous solution of mannan.EFFECT: invention enables to obtain polysaccharide-containing matrices with a long shelf life, used to include a wide range of biologically active substances
Method for comprehensively extracting algal polysaccharide, algal polyphenol and fucoxanthine
CN108034008
The invention belongs to the technical field of extraction of marine natural products, and discloses a method for comprehensively extracting algal polysaccharide, algal polyphenol and fucoxanthine. The method comprises the following steps: pretreating raw materials, then carrying out extraction with the addition of water, separately collecting the extract and the residue, carrying out reduced pressure concentration on the extract, then adding ethyl alcohol until the concentration is 20-30%, collecting the precipitate, drying the precipitate to obtain fucoidan, continuing adding ethyl alcohol into the supernatant until the final concentration is 70-80%, collecting the obtained precipitate, drying the obtained precipitate to obtain algal polysaccharide sulfate, adding 80-90% ethyl alcohol into the residue obtained after the extraction of algal for extraction, collecting the alcohol extract, carrying out reduced pressure concentration on the alcohol extract, carrying out standing overnight at a temperature of 4 DEG C, carrying out filtering, collecting the obtained precipitate, carrying out freeze-drying on the precipitate to obtain a fucoxanthine crude product, treating the supernatant after the filtering by using macroporous adsorption resin, carrying out washing for removing impurities, carrying out elution with 50-90% ethyl alcohol, carrying out reduced pressure concentrationon the obtained eluant for recovering the ethyl alcohol, and carrying out freeze-drying to obtain the algal polyphenol. The method provided by the invention has a simple technological process, various products can be obtained simultaneously through one-time comprehensive extraction and separation, and the high-valued comprehensive utilization of algae is realized.
Preparation method of high sulphate group fucoidan polysaccharide
CN108003256
The invention provides a preparation method of high sulphate group fucoidan polysaccharide, and belongs to the technical field of active polysaccharides. The method solves the problems that in the process of extracting the fucoidan polysaccharide in the prior art, extraction liquid has many impurities; the polysaccharide leaching efficiency is low; the product purity is not high; the polysaccharide decomposing is caused by high temperature, strong acid or strong alkalinity environment in the preparation process; active groups such as sulphate groups are lost, and the like. According to the method, algal is used as a raw material; firstly, the algal is subjected to ultrafine crushing treatment, so that the algal cells are subjected to wall breaking; the polysaccharide leaching rate is increased; the extraction time is reduced; then, a water extraction method is used for extracting the fucoidan polysaccharide; the polysaccharide decomposing and the sulphate group activity loss can be reduced; the natural ingredients and activity of the fucoidan polysaccharide can be greatly remained; finally, the extraction liquid is subjected to protein removal by trichloroacetic acid, sodium alginate impurity removal through ethanol precipitation, and polysaccharide precipitation by ethanol, so that the high-purity and high sulphate group algal polysaccharide is obtained. The purity of the fucoidan polysaccharide prepared by the method can reach 60 to 70 percent; the organic sulfate radical content reaches 20 to 30 percent.
DRY EXTRACT FROM FUCUS ALGAE WITH ANTIOXIDANT ACTION AND THE METHOD OF ITS PRODUCTION
RU2650808
FIELD: medicine; pharmaceuticals. SUBSTANCE: invention relates to medical and therapeutic-preventive practices, to the chemical-pharmaceutical, food, and cosmetic industries. Difference in obtaining the claimed dry extract from fucus algae, which has an antioxidant effect,is that algae are used: Fucus vesiculosus, Fucus distichus and Fucus serratus; extraction of algae is carried out with a mixture of fructose, glucose, sucrose and water (in a molar ratio of 1:1:1:11) 20 % and water 80 % using microwave radiation with a power of 500-600 W for 10-15 minutes at a temperature of 40-60 °C, then the enzyme protosubtilin is added to the reaction mixture at a rate of 1-3 g per 100 g of extract and fermentation is performed with ultrasonic treatment at a frequency of 42 kHz at a temperature of 40 °C for 30 minutes; at the end of the process the enzyme is inactivated, the algae cake is separated, the liquid extract is treated with a flocculant at the rate of 0.5-1.5 g per 100 g of the extract, the precipitate of the flocculant is separated. As an extractant, a mixture of proline, malic acid and water (in a molar ratio of 1:1:3) 20 % and water 80 % is used. As a raw material for the production of dry extract from fucus algae, which has an antioxidant effect, fresh, frozen or dry algae thalli can be used. Hydromodule for fresh and frozen algae can be 1:1, for dry - 1:5. As a flocculant, chitosan or microcrystalline cellulose (MCC) can be used. Drying of the liquid extract can be carried out lyophilically or by means of a spray dryer. Dried extract from fucus algae, which has the antioxidant effect obtained by the above method, is a complex of biologically active substances (BAS) of the following composition: fucoidan 50-60 g, alginic acid 1-5 g, polyphenols 25-35 g, free amino acids 50-100 mg, ascorbic acid 0.5-1 g in 100 g of dry extract. EFFECT: claimed invention ensures achievement of the indicated technical results, namely, ensuring the most complete extraction of biologically active substances of fucus algae and obtaining a dry extract from fucus algae with enhanced antioxidant activity and improved solubility in water.
Method for extracting fucoidan from kelp by using ultrasonic waves
CN107722129
The invention relates to the technical field of deep processing of marine resources, and particularly discloses a method for extracting fucoidan from kelp by using ultrasonic waves. According to the method, kelp is used as a raw material; and the method comprises: washing kelp, drying, and crushing; carrying out degreasing treatment on the crushed kelp; carrying out centrifugation on the degreasedkelp, collecting the precipitate, and drying to obtain kelp powder; mixing the kelp powder and water, adding composite enzyme comprising cellulase, hemicellulase, protease and pectinase, and carryingout enzymolysis; ultrasonically extracting the kelp powder obtained after the enzymolysis; removing the supernatant from the ultrasonically treated solution, concentrating, and adding ethanol; and carrying centrifugation on the concentrated fucoidan, and washing the separated precipitate by using ethanol to obtain the crude fucoidan. According to the present invention, the method has advantages of simple operation, short extraction time and low energy consumption, wherein the extraction work is performed at the low temperature, such that the damage of the high temperature on the extracted component can be avoided while the precipitation of the pigment during the extraction can be reduced.
Extraction method of sargassum fusiforme phlorotannins
CN107550944
The invention discloses an extraction method of sargassum fusiforme phlorotannins. The extraction method comprises steps as follows: pretreatment of raw materials, extraction of phlorotannins and purification of phlorotannins. The extraction method has beneficial effects as follows: the steps are simple, few technological processes are required, polyphenol extraction speed is high, extraction effect is good, a crude polyphenol product solution contains few alcohol-soluble impurities, and content of pigment composition and fucoidan is low; with addition of active peptides, extraction efficiencyis improved, solution viscosity is increased, and polyphenol oxidation is avoided; by means of NKA-9 macroporous resin, impurities such as polysaccharide, protein and the like in the extracting solution are further removed, and product purity is improved.
Brown algae polyphenols extracted from sargassum fusiforme
CN107412277
The invention discloses brown algae polyphenols extracted from sargassum fusiforme. Extraction comprises the steps of raw material pretreatment, extraction of the brown algae polyphenols and purification of the brown algae polyphenols. The extraction has the beneficial effects as follows: the steps of brown algae polyphenol extraction are simple, few the technological processes are adopted, the polyphenol extraction speed is high, the extraction effect is good, few alcohol soluble impurities are contained in a crude polyphenol product solution, and content of pigment composition and fucoidan is low; the extraction efficiency is increased by means of added active peptides, the viscosity of a solution is improved, and oxidation of the polyphenols is avoided; impurities such as polysaccharides, proteins and the like in an extract solution are further removed by NKA-9 macroporous resin, and the purity of the product is improved.
Energy-saving and high-efficiency method for extracting soluble organic matters from kelp blanching water
CN107382666
The invention relates to an energy-saving and high-efficiency method for extracting soluble organic matters from kelp blanching water. The energy-saving and high-efficiency method comprises the following main eight steps: sprinkling in the conveying process of a fresh kelp raw material, blanching the raw material in a blanching pot after sprinkling, performing high-temperature nanofiltration on a sprinkling liquid after purification of the sprinkling liquid, performing three-section extraction on the sprinkling liquid, salting the kelp raw material after the three-section extraction to obtain a salted finished kelp product, performing ultrafiltration purification separation on a multi-stage sprinkling liquid, purifying a mannitol product, purifying a fucoidin product and the like. By the treatment method provided by the invention, a one-time blanching water heating and multi-stage heat recovering process is adopted, heat obtained during one-time heating is successively utilized through multi-stage blanching, and the extracting process is speeded up, so that the efficiency is improved and the energy is saved; moreover, a purifying process is optimized, and mannitol with the purity not lower than 99.95% and fucoidan with the purity not lower than 99.8% are obtained.
METHOD FOR WATER-SOLUBLE COMPONENTS EXTRACTION FROM MARINE BROWN ALGAE ENRICHED BY FUCOIDAN AND IODINE
RU2016104796
FIELD: pharmacology.SUBSTANCE: method for marine brown algae processing, including crushing of raw materials, homogenization of the mixture of raw materials a in solution, followed by extraction of water-soluble biologically active substances by filtration and drying, the raw material is pre-washed with water saturated with ozone, the raw material is homogenized in a solution that is water with calcium chloride at its concentration of 0.25 to 1% in the solution, additional grinding of the crushed algae is carried out in a mixture of raw materials in a solution at revolutions of rotary-pulse homogenizer, constituting 10 to 50% of the maximum engine revolutions, while 10 to 30% of the total amount of the mixture is loaded by batches during the preliminary homogenization, after completion of loading and preliminary homogenization, the rotation speed of the rotary-pulse homogenizer is increased and the mixture is homogenized for up to 5 hours at a mixture temperature of not more than 75°C. After homogenization, the homogenate is cryodestructed at a temperature from minus 18 to minus 20 degrees for 20-48 hours, for which the homogenate is placed in containers of 0.5 to 10 l, after cryodestruction, the frozen homogenate is thawed, after homogenate separation, liquid is separated from the precipitate, the precipitate is filtered under pressure and the resulting liquid is mixed with the liquid obtained in the previous step, the liquid phase is concentrated on a membrane cell, calcium chloride is removed, the precipitate and the liquid are dried.EFFECT: above method promotes production of products with an increased content of biologically active substances.
Extraction and separation process of fucoidan sulfate
CN106916234
The invention discloses an extraction and separation process of fucoidan sulfate. The process includes the steps of filtrate preparing, ultra-filtration, wash-filtration, and post-treatment. In the process, an ultra-filtration membrane group is used for performing the ultra-filtration twice, so that a problem of water mixing during a conventional process with electrodialysis is solved; generally, desalinization rate is 75-80% in electrodialysis, but the method in the invention can reach 95% in desalinization rate, so that the method is high in separation purity, is a pure-physical process without any chemical reactions, and is free of secondary pollution. The method is carried out at low temperature and is very suitable for separation of high-heat sensitivity components. The separation process has no vapor consumption, wherein electric consumption is only 1/2 in electrodialysis, thereby significantly reducing operation cost. The ultra-filtration membrane group is operated in an auto-control continuous treatment manner, and the wash-filtration is on-line auto-wash-filtration, so that the process has high automation degree, and is reduced in labor intensity and labor cost.
Method for extracting fucoidan sulfate
CN106832022
The invention discloses a method for extracting fucoidan sulfate. The method comprises the following steps: cutting kelp into uniform kelp shreds, treating the kelp shreds with ethanol, cooking and soaking the kelp shreds in water, then performing acid extraction and microwave countercurrent extraction to obtain a crude kelp fucoidan sulfate extract, then adding calcium chloride, performing multi-stage filtration to obtain a filtrate, performing ultrafiltration and concentration on the filtrate to obtain a concentrate, performing alcohol precipitation on the concentrate, drying and crushing to obtain the fucoidan sulfate. The method is simple, the using amount of an organic solvent is less, few steps are adopted, the cost is low and the kelp can be recycled, so that the method is suitable for industrial production; the content of the obtained fucoidan sulfate is 2.0-2.8%, wherein the fucose content is 75.2-80.8% and the sulfate radical content is 55.6-75.4%.
Method for extracting fucoidan through biological enzymolysis process
CN106832041
The invention belongs to the technical field of fucoidan extraction and provides a method for extracting fucoidan through a biological enzymolysis process. The method comprises the following steps: grinding kelp into slurry; soaking; adding acidic cellulase and hemicellulase; adding alkaline pectinase; adding protease; adding sodium chloride and water and reacting, thereby acquiring sodium alginate jelly; adding modified attapulgite; centrifuging by using a centrifugal machine; and adding ethyl alcohol and dewatering, thereby acquiring solid-phase fucoidan. According to the method for extracting fucoidan provided by the invention, the biological enzymolysis process is adopted; on the basis of the structure and ingredients of kelp cytoderm, a defined amount of related enzyme is added in stages, so that the cytoderm is thoroughly split; the fucoidan therein is fully released, so that the extraction rate of the fucoidan is increased; the modified attapulgite is added for decolorizing and removing fishy smell in an extraction process, so that the appearance of the product is cleaner; and besides, the extraction steps are simplified, the use of the chemical raw materials is reduced and the purpose of environmental protection is achieved.
Method for producing higher-pyrity and depolymerizing fucoidan extracted from brown algae
KR20160149748
The present invention relates to a fucoidan preparation method including: a pretreatment step of drying and desalting brown algae; an extraction step of extracting a fucoidan extract from the pretreated brown algae; a purity increasing step of removing alginic acid by precipitation from the extracted fucoidan extract; a depolymerizing step of depolymerizing the fucoidan by adding acid to the purity-increased fucoidan extract; and a purification step of removing other foreign matter from the depolymerized fucoidan extract. According to the present invention, a fucoidan element can be extracted from the brown algae, the purification yield of the fucoidan can be increased through the purity increasing and depolymerizing steps, and fucoidan can be provided that has a molecular weight facilitating absorption into the human body.
Fucoidan powder preparation method
CN106188330
The invention discloses a fucoidan powder preparation method. The method is characterized by including the following steps that brown algae are obtained to obtain a brown alga extraction solution, the extraction solution is concentrated, the concentrated brown alga extraction solution is subjected to primary ethanol precipitation, the primary ethanol precipitation solution is subjected to centrifugation to obtain a centrifugal solution, supernatant of the centrifugal solution is subjected to secondary ethanol precipitation, precipitate is obtained and subjected to gradient drying, and then the precipitate is smashed to obtain dry fucoidan powder. The fucoidan powder preparation method is easy to operate, large in processing amount, high in application safety and suitable for large-scale industrial production.
Method for preparing sea cucumber fucoidan and sea cucumber glycoprotein
CN105695545
The invention provides a method for preparing sea cucumber fucoidan and sea cucumber glycoprotein. According to the method, acaudina molpadioides serves as the raw material, a mild stepped enzymolysis processing method is mainly adopted, an ultrafiltration membrane is utilized to separate out a mixture of crude polysaccharide and glycoprotein, then separation, purification and preparation of the sea cucumber fucoidan and the sea cucumber glycoprotein are achieved through a Q-Sepharose-F-F ion exchange column and an Sephadex G-150 sephadex column, a prepared sea cucumber fucoidan product is fawn, and the sea cucumber glycoprotein is faint yellow. According to the method, a co-production method is adopted, main ingredients such as polysaccharide and glycoprotein in the acaudina molpadioides can be prepared in a jointed mode, emission of waste is reduced in the extraction process, and production cost is lowered.
Method for extracting fucoidan by using Sargassum kjellmanianum
CN105273104
The invention discloses a method for extracting fucoidan by using Sargassum kjellmanianum. According to the method, the Sargassum kjellmanianum is selected and soaked in water, the pH value of a soaking liquid is adjusted to range from 4 to 5, solids are leached out after heating and acid extraction, then the pH value of an extraction liquid is adjusted to range from 6 to 7, centrifugation is performed, a supernate is taken and passes through a resin column taking polytetrafluoroethylene powder as filler, and a filtered solution is collected; the filtered solution is separated through a nano-filtration membrane, and a trapped solution is collected; the trapped solution is subjected to spray-drying or freeze-drying or alcohol precipitation, precipitates are dried, and a dried fucoidan product is prepared. The extracting method is simple to operate, the process steps are reasonable, and the equipment investment is low. The purity of the fucoidan obtained through extraction with the method is high, and the content of the effective component is more than 85%.
Method for preparing fucoidan by utilizing sargassum
CN105061633
The invention discloses a method for preparing fucoidan by utilizing sargassum. Fresh sargassum serves as a raw material and is cleaned, dried and smashed and then screened to prepare sargassum powder, crude proteoglycan protein mixture is extracted from the argassum powder by adopting an acid-extraction method, the crude proteoglycan protein mixture is purified through a CaCl2 precipitation method; after protein is removed from the obtained purified proteoglycan protein mixture in a trichloroacetic acid-Sevage combined method, an anion exchange chromatography purification technology is adopted, so that polysaccharide exists in a crossing peak, the activity of the polysaccharide can be maintained well, and the purity of the obtained polysaccharide is high. The method is rational in process, high in handling capacity, simple to operate and favorable for mass production, and the content of total sugar and sulfated groups in the finally obtained fucoidan is high.
Method for comprehensively extracting fucoxanthin and fucoidan from sargassum
CN105037580
The invention discloses a method for comprehensively extracting fucoxanthin and fucoidan from sargassum. The method for comprehensively extracting fucoxanthin and fucoidan from the sargassum is characterized by comprising the following steps: unfreezing fresh sargassum or frozen sargassum at room temperature, washing with clear water, and controlling moisture content of the sargassum to be 50-90%; adding the sargassum into ethanol water, shaking up, wrapping a conical flask with tinfoil, putting the conical flask into a shaking table, extracting for 1-8 hours at normal temperature, and filtering to obtain filtrate; carrying out vacuum concentration on the filtrate until a large amount of black substance is separated out, and continuously carrying out vacuum concentration until red solid, namely high-purity fucoxanthin, is separated out; putting filter residue into a Soxhlet extractor, refluxing with ethanol water, extracting for 1-5 hours at the temperature of 60-90 DEG C, soaking and extracting the extracted solid matter in water for 3-8 hours at the temperature of 60-90 DEG C, filtering, centrifuging, taking supernate, carrying out vacuum concentration, adding ethanol, uniformly mixing, filtering, taking filter residue, washing with ethanol, and drying, so that the fucoidan is obtained. The method for comprehensively extracting the fucoxanthin and fucoidan from the sargassum has the advantages that raw material utilization rate is high, the extraction efficiency is high, and the method is simple and feasible.
Extracting and purifying method of fucoidan from fucus vesiculosus
CN102936293
The invention relates to an extracting and purifying method of fucoidan from fucus vesiculosus. The extracting and purifying method comprises the following steps of: S1, with sea-tangle waste generated after mannitol is extracted as raw material, adding deionized water with pH of 5-6 according to a proportion, intermittently extracting for 3-6 times under the microwave condition of 2450MHz and 600-1200W, wherein each extraction is carried out for 2-7min and a certain proportion of deionized water is replenished every time; S2, centrifuging an extraction solution to obtain supernate, concentrating the supernate and then adding a certain proportion of alcohol, generating a sediment, centrifuging again to obtain supernate; S3, cooling the supernate to 5-10DEG C, slowly adding a certain proportion of alcohol, seriously stirring, gradually generating a white particle sediment; and S4, collecting the sediments, washing by using absolute ethyl alcohol, and drying to obtain the fine particle fucoidan from fucus vesiculosus.
Method for separating and purifying sea cucumber polysaccharide
CN102532340
The invention provides a method for separating and purifying sea cucumber polysaccharide. The method comprises the following steps of: 1, separating, namely performing two aqueous phase extraction on the sea cucumber polysaccharide to obtain an extraction upper phase and an extraction lower phase; and 2, performing aftertreatment, namely desalting the extraction upper phase obtained in the step 1 and drying to obtain fucoidan isolatedfrom sea cucumber, and desalting the extraction lower phase obtained in the step 1 and drying to obtain sea cucumber chondroitin sulfate so as to separate and purify the sea cucumber polysaccharide. A novel separation technology, namely the two aqueous phase extraction technology is applied to the separation and purification of the sea cucumber polysaccharide; and the method has the advantages of mild condition, convenience in operation, low requirement on equipment, and low cost; and amplification is easy to realize, a product is high in purity, and the defects in the conventional method for separating and purifying the sea cucumber polysaccharide are overcome.
FLOCCULANT DERIVED FROM ENVIRONMENTALLY-FRIENDLY NATURAL PRODUCT USING OKINAWA MOZUKU (CLADOSIPHON OKAMURANUS) AS RAW MATERIA
JP2012071293
PROBLEM TO BE SOLVED: To develop a more effective water treatment agent and flocculant/precipitant by utilizing a natural resource such as seaweeds and particularly an industrially economical and environmentally-friendly water treatment agent and flocculant/precipitant, which are free from any problem by using residues of Cladosiphon okamuranus after extraction of components efficacious for pharmaceutical and healthy food products.SOLUTION: The water treatment agent is produced by crushing Cladosiphon okamuranus in water, extracting water-soluble components such as fucoidan or the like under acidic condition by adding an acid to the obtained aqueous solution, and using the resulting residues as a raw material.
Method for synthetically utilizing kelp to extract and purify fucoxanthine and fucoidan organosulfate
CN102321052
The invention discloses a method for synthetically utilizing kelp to extract and purify fucoxanthine and fucoidan organosulfate, which comprises the following steps that: (1) cleaned kelp is pulped, the hexane or sherwood oil is used for extraction through continuous countercurrent, and kelp pulp slag and extracting liquid are obtained through filtering; (2) solvents are completely volatilized, the absolute ethyl alcohol is added for dissolution, the filtering is carried out, the obtained filter liquid is crude fucoxanthine extracting liquid, in addition, ethanol water solution is added to the obtained pulp slag for ultrasonic extraction and filtering, the filter slag is added with water for ultrasonic extraction and filtering, and the obtained extracting liquid is crude fucoidan organosulfate extracting liquid; and (3) resin columns are mixed on crude the fucoxanthine extracting liquid and the crude fucoidan organosulfate extracting liquid, and the elution, the separation and the purification are carried out.; The method disclosed by the invention has the advantages that a large number of solvents are saved, the inorganic salt mass in the products is reduced, few efficient ingredients is lost, the production efficiency can be greatly improved, the production cost is reduced, and the raw materials can be more fully utilized.
METHOD FOR COLLECTING FUCOIDAN-CONTAINING EXTRACT FROM ALGA BODY OF BROWN ALGA (SCIENTIFIC NAME: ECKLONIA CAVA KJELLMAN)
JP2011093865
PROBLEM TO BE SOLVED: To solve the problems associated with a conventional method for obtaining an extracted component from EcKLonia cava Kjellmen, e.g. wherein a large amount of an extract relative to the alga body weight of EcKLonia cava Kjellman cannot be obtained due to complicated extraction method. ;SOLUTION: A viscous extract containing fucoidan is collected together with hot water through steps of: collecting brown alga (scientific name: EcKLonia cava Kjellman); shredding the alga and resting or culturing the same under appropriate moisture and temperature conditions so that a large amount of the viscous extract can be released; and kneading a fresh alga body, thereby forcing the alga to release mucus contained in spores.
COMPLETE PROCESSING METHOD OF SEA TANGLE (LAMINARIA JAPONICA)
KR20090127785
PURPOSE: A method for processing kelp, and th kelp product processed by the method are provided to utilize the total parts of kelp and to induce no pollution. CONSTITUTION: A method for processing kelp comprises the steps of putting raw kelp or the cut kelp into an extraction tank, putting water to it and extracting it for 20-30 min to obtain an extract; extracting the chlorophyll and fucoxanthin contained in the remaining part with ethanol with stirring, removing ethanol under reduced pressure, and concentrating it to obtain a concentrate; heating the residue remaining after freeze-drying the concentrate with weak acidic water for 24 hours to extract fucoidan, concentrating it and freeze-drying it; extracting the alginic acid contained the residue the diluted calcium carbonate hot water for 2-3 hours, concentrating it and neutralizing it; precipitating alginic acid with ethanol and drying it; and mixing the fiber contained in the residue with a kelp powder to make a kelp granule.
Method for extracting fucoidan for brown seaweed
CN101619104
The invention relates to a fucoidan extract method, in particular to a method for extracting fucoidan for brown seaweed, comprising the following steps: 1. desalting: adopting fresh brown seaweed or unfreezed frozen brown seaweed to soak in pure water to remove salt; 2. enzymolysis: adding enzyme in the desalted brown seaweed to perform enzymolysis at 40-60 DEG C for 1-3h; 3. abstraction: adding calcium chloride in enzymatic hydrolyzate obtained by enzymolysis, and heating the solution to 90-100 DEG C for 1-4h to obtain the fucoidan extract. The invention is characterized of simple operation, high application security, high extraction efficiency, high extraction purity and the like and the extraction process is applicable to factory mass production.
APPARATUS FOR AND METHOD OF EXTRACTING AND PURIFYING FUCOIDAN
JP2009084460
PROBLEM TO BE SOLVED: To eliminate loss of seaweed raw materials when handling the same for extracting fucoidan in the form of a viscous substance, and to produce a large amount of high-quality fucoidan. ;SOLUTION: In an apparatus, three tank structures, which are designated as a funnel, an extraction tank and a server tank, are connected as shown in Fig.1. The funnel and the extraction tank is connected to each other through an internal structure comprising an internally contacting screen frame, as shown in Fig.2. By operating valves built on the tanks, fucoidan contained in seaweeds is extracted from a seaweed powder under heating and subsequently subjected to solid-liquid separation and purification by sedimentation. A method for extracting and purifying fucoidan is also provided.
Method for Producing Fucoidan, Fucoidan, and Fucoidan-Containing Composition
US2009105190
The present invention provides a method for producing high molecular-weight fucoidan, which is expected to be promising as a cosmetic composition or an agent in the field of dermatology, and a high molecular-weight fucoidan-containing composition. A component containing fucoidan having a weight average molecular weight of 1,000,000 to 2,000,000 obtained through the steps of performing hot water extraction of a mozuku alga body of the genus Cladosiphon at pH 6.0 or higher, which ranges from neutral to alkaline, removing low-molecular weight compound through ultrafiltration, and adjusting a final pH to 6.5 or higher is effective as a cosmetic composition or an agent in the field dermatology, and is also excellent in storage stability.
METHOD FOR PRODUCING FUCOIDAN
JP2008266528
PROBLEM TO BE SOLVED: To provide a method for producing a fucoidan by which the extraction efficiency of the fucoidan from seaweeds such as a sea tangle, Nemacystus decipiens and Sargassum horneri is raised and to further provide a method for producing the fucoidan by which there is no problem such as corrosion of production equipment or an increase in the number of production steps and production cost can be reduced because an acid, a salt etc. , are not used as an extractant. ;SOLUTION: The method for producing the fucoidan comprises a step of pulverizing the dried seaweed and affording powder of the seaweed, a step of adding 5-100 pts.wt. of water to 100 pts.wt. of the powder of the seaweed and preparing a composition comprising the powder of the seaweed and water, a step of kneading the resultant composition using an extruder in which the maximum temperature in the cylinder is 60-180[deg.]C and a step of extracting the fucoidan.
SIMULTANEOUS PRODUCTION METHOD OF FUCOXANTHINE AND FUCOIDAN
JP2008255231
PROBLEM TO BE SOLVED: To provide a method for simultaneously and efficiently producing fucoidan and fucoxanthine. SOLUTION: The simultaneous production method of fucoxanthine and fucoidan comprises performing extraction treatment of previously crushed Cladosiphon okamuranus at 10-50°C with ethanol of practically 65-85 vol.% concentration in extraction, rendering the extraction liquid after solid-liquid separation to resin adsorption treatment, obtaining fucoxanthine from an eluate obtained by eluting the adsorbing resin with ethanol of 85-100 vol.% and extracting the extraction residue after solid-liquid separation with an organic acid on a condition within a range of pH2-pH6, and obtaining fucoidan from the extraction liquid.
METHOD FOR CONTINUOUS EXTRACTION OF PHYSIOLOGICALLY ACTIVE SUBSTANCES FROM BROWN ALGAE
KR20040105523
PURPOSE: Provided is a method for continuous extraction of physiologically active substances from brown algae, which reduces fucoxanthine loss, permits production of more hygienically preferable products, is cost- and time-efficient, and prevents environmental pollution. CONSTITUTION: The method for continuous extraction of physiologically active substances comprises the steps of: washing living natural brown algae to remove impurities and salt; mixing the washed brown algae with an organic solvent and weak alkaline solution to extract fucoxanthine, alginic acid, fucoidan and laminarin, continuously; and carrying out centrifugal separation of the extracts and concentration thereof to provide freeze-dried functional food materials.
PROCESS FOR PRODUCTION OF FUCOIDAN EXTRACT
JP2007332320
PROBLEM TO BE SOLVED: To provide a production process to easily obtain a fucoidan extract from algal raw materials containing fucoidan such as brown seaweed. ;SOLUTION: The extraction process to obtain the fucoidan extract liquid includes a heating process of algal raw materials containing fucoidan such as brown seaweed at 40-100[deg.]C, a shaking process of the algal raw materials by immersing in an aqueous solution of 5 mass% or less of a salt at 0-40[deg.]C, a shaking extraction process of the aqueous solution after the shaking process as the fucoidan extract, an addition process of a neutral salt to the algal raw materials, and obtaining the fucoidan extract as a blowing down liquid from the algal raw materials by the addition process.
METHOD FOR PROCESSING SEAWEED CONTAINING FUCOIDAN
JP2006340696
PrOBLEM TO BE SOLVED: To enhance extracting rate of an extract containing fucoidan. ;SOLUTION: The invention relates to the method for extracting the extract containing fucoidan comprising a soaking process soaking the seaweeds containing fucoidan in a solution added with bittern as a preliminary process, and a miniaturization process of the soaked seaweeds. The extraction of alginic acid contained in the seaweeds is prevented by minerals such as Mg ion or the like contained in the bittern through the preliminary process which soaks the seaweeds containing fucoidan in the aqueous solution added with the bittern. The rate of extraction of the extract is enhanced by inhibiting elution of the extract before following process of miniaturizing the seaweeds and extracting the extract containing fucoidan.
METHOD FOR EXTRACTING FUCOIDAN
JP2006160862
PROBLEM TO BE SOLVED: To solve the problems that the complexity of conditions for separating and purifying fucoidan allows the production of only a limited amount of fucoidan, that production equipment needs to be made of an anticorrosive material which is expensive and difficultly workable, that when a strong acid is used as an extracting solvent, the produced fucoidan needs to be finally neutralized with a strong base, which causes an increase in the number of production steps and an increase in an impurity content, and that the efficiency of extraction of fucose from fucoidan is extremely low, and a like problem. ;SOLUTION: The method for extracting fucoidan comprises lyophilizing wakame sprouts washed with seawater or saline water, extracting the lyophilized sprouts with hot water or an acid for an extraction time in the range of 0.5 to 72 h, and adjusting hot water used to extract the ground sprout to a pH in the range of 2 to 3 to extract colorless, odorless high-purity fucoidan. Alternatively, the method is one in which a fucoidan-like polysaccharide composite obtained by extracting a sprout powder prepared by freeze-drying wakame sprouts washed with running water and grounding the freeze-dried sprouts contains fucose and which comprises the step of lyophilizing washed sprouts and the step of grinding the lyophilized sprouts.
MANUFACTURING METHOD OF CONCENTRATED LIQUID FOR EXTRACTING FUCOIDAN FROM POWDER OF DRIED NEMACYSTUS DECIPIENS
JP2005145989
PROBLEM TO BE SOLVED: To provide an extracting means for ensuring a yield very close to 24.2%, the fucose content Nemacystus decipiens naturally has, by increasing extraction efficiency of fucose from the raw material Nemacystus decipiens by leaps and bounds. ;SOLUTION: The method comprises extracting fucoidan from powdery products obtained by crushing and processing dried Nemacystus decipiens. The powder Nemacystus decipiens obtained by crushing and processing the dried Nemacystus decipiens is kneaded with water 25 times the weight thereof and the kneadate is boiled with agitation in an agitating hot-water extractor or a high-pressure extraction pot for 60-120 min at 80-90°C after adding an appropriate amount of water (diluted with an alcohol as occasion demands). The boiled product is transferred to an alcohol extraction tank, cleared of the alcohol and subjected to filtration and extraction to give a stock solution. The stock solution is subjected to separative purification by an ultrahigh-speed centrifuge and is subsequently concentrated by 4-8 times by a concentration machine to give a fucoidan-containing concentrated liquid. The fucose content in the fucoidan-containing concentrated liquid manufactured by the method corresponds to the yield of 21.6% based on the weight of the raw material powder Nemacystus decipiens used.
CONVERSION OF BROWN ALGAE LIVING ALONG COASTAL WATERLINE TO EXTRACT
JP2002265380
PROBLEM TO BE SOLVED: To convert, in a low-molecular condition, the active ingredient in brown algae grown along coastal waterline and hopeful of higher functionality to extract so as to take the whole extract component into the human body to ensure the above sea algae to be rated as a functional chemical substance in view of the fact that although the polysaccharides in sea algae are important as mammal's immunological function activator, the polysaccharides in a natural condition constitutes a polymer complex, therefore even if the natural sea algae are taken into the human body as they are, substantially the whole quantity thereof is excreted. SOLUTION: The objective extract is the main component of the sea algae living coastal waterline including brown algae, Fucus evanescens, Undaria pinnatifida, Ascophyran and Sargassum Thunbergii. This extract is obtained by solution extraction with citric acid or disodium phosphate of the carbohydrates of gel-like polysaccharide connective tissue zone indigestible in the human body including gel-like seaweed polysaccharides, alginic acid, fucoidan, sargassum and ascophyn after converted to the corresponding monosaccharides in a free form.
SIMPLE PRODUCTION METHOD OF FUCOIDAN-CONTAINING EXTRACT
JP2002220402
PROBLEM TO BE SOLVED: To extract simply a fucoidan-containing extract which does not substantially contain arsenic from the starting brown algae. SOLUTION: In the extraction of a fucoidan from a staring material, an arsenic is not eluted. The fucoidan-containing extract is produced from the starting brown algae material by a method comprising (A) a step of treating the material with an alkali-containing solvent of 0.1-300 pts.wt. based on the material; (B) a step of recovering the extract from the treated solution, wherein the solvent is preferably a sodium hydroxide or potassium hydroxide of 0.01M-1M and it contains a hydrogen peroxide of 0.005-5%; and the step of treating with the solvent, preferably at 50-100 deg.C for 10-120 minutes.
NEW ULTRA-LOW-MOLECULAR NEMACYSTUS DECIPIENS ESSENCE
JP2002165579
PROBLEM TO BE SOLVED: To provide a method for producing ultra-low-molecular Nemacystus decipiens (scientific name) essence for recovering and preventing by Nemacystus decipiens essence component many intractable diseases involved in at present, such as prevention and control of various circulatory geriatric diseases and every kind of cancer, functional recovery of internal organs such as the liver, prevention of infection and control of HIV, through infiltrating the ultra-low- molecular Nemacystus decipiens essence into body fluid through drinking and from the skin, and to provide a method for producing minute granular fucoidan with high purity. SOLUTION: This method for producing ultra-low-molecular Nemacystus decipiens essence is improved through e.g. subjecting raw Nemacystus decipiens to boil essence extraction, hydrolysis and filtration which acidic water (citric acid water). The method for producing minute granular fucoidan with high purity comprise making use of the ultra-low-molecular Nemacystus decipiens essence thus obtained.
PRODUCTION OF FUCOIDAN
JPS6487601
PURPOSE:To obtain fucoidan suitable as a flocculant, thickening agent or surface active agent, having high viscosity and excellent stability in precipitating fucoidan from an extracted solution of brown algae, by adding an agent such as an alkali salt of carboxylic acid or an alkali salt of polymerized phosphate. CONSTITUTION:In precipitating fucoidan from an extracted solution of brown algae with an alcohol or washing the precipitate with an alcohol or reprecipitating with an alcohol, an agent selected from an alkali carboxylate or an alkali salt of polymerized phosphate is added to give the aimed fucoidan. The extraction temperature is about 40 deg.C and the amount of water added is preferably about 5-10 times as much as the weight of the brown algae.
PRODUCTION OF NEMACYSTUS DECIPIENS EXTRACT
JPH111437
PROBLEM TO BE SOLVED: To obtain the subject extract useful as e.g. a medicine by boiling Namecystus decipiens in an aqueous alcohol solution to effect extraction of all of the active ingredients in the order of ultrafine molecules and enable percutaneous of intracellular direct infiltration of the active ingredient through intravenous drip injection. SOLUTION: Nemacystus decipiens (e.g. Chordaria flagelliformis) is boiled in an aqueous solution of an alcohol such as ethyl alcohol nontoxic to human body, the resultant Nemacystus decipiens mixed liquor is then filtered to obtain the objective Nemacystus decipiens extract which is useful as a cosmetic, health food/drinking water, etc., because all of the active ingredients thereof are intraintestinally digestible when taken in vivo. This extract contains chlorophyll c1 , chlorophyll c2 , etc., as decomposition products of chlorophyll a strucutre, ß-carotene and violaxanthin, etc., as decomposition products of cartenoid, alginic acid, and fucose and galactose, etc., as decomposition products of fucoidan- constitutive sacchardies.
EXTRACTION OF FUCOIDAN
JP2000239302
PROBLEM TO BE SOLVED: To provide an extraction method that gives fucoidan of a relatively high average molecular weight with no problem of corrosion of the facility and the like in the production installation. SOLUTION: When fucoidan is extracted from a seaweed, Cladosiphon okamuranus, an organic acid is used in an amount of 1-100 g/1,000 g of the seaweed and the pH in the extraction process is adjusted in the range from 2 to 6.
EXTRACTION OF FUCOIDAN
JP2000236889
PROBLEM TO BE SOLVED: To extract fucoidan having anticholesterol action, anticancer action and anti-AIDS virus action, by swelling Cladosiphon okamuranus with an organic acid salt followed by carrying out enzymatic treatment using a fucoidan splitting enzyme. SOLUTION: Fucoidan is extracted by the following process: 1,000 g of Cladosiphon okamuranus is swollen with 1-100 g of an organic acid salt and then subjected to enzymatic treatment at 25-95 deg.C for 0.5-48 h with a fucoidan splitting enzyme such as cellulase produced by Aspergillus niger, thereby efficiently obtaining the objective fucoidan having pharmacological activities including anticholesterol action, blood clearing action, anticoagulant action, anticancer action, anti-AIDS virus action, and anti-gastroulcer action.
EXTRACTION OF FUCOIDAN
JP2000239301
PROBLEM TO BE SOLVED: To provide an extraction method that gives fucoidan of a desired average molecular weight in high yield with no problem of corrosion of the facility and the like in the production installation. SOLUTION: In this extraction process, fucoidan is extracted from the seaweed, Cladoiphon okamuranus, with pressurized hot water in an autoclave. The extraction temperature is set to 100-120 deg.C, the extraction pressure is set to 1.0-2.0 atmospheric pressure and the extraction is continued for 0.2-16 hours.
IMPROVEMENT OF QUALITY OF MOZUKU EXTRACT AND MOZUKU EXTRACT
JPH10191940
PROBLEM TO BE SOLVED: To obtain fucoidan-contg. extract which is substantially tasteless, odorless and pale and is useful for treatment of gastric diseases, etc., from mozuku (Nemacystus decipiens (SURINGAR) KUCHUCK) by subjecting the mozuku or its extraction liquid to a hydrogen peroxide treatment. SOLUTION: The fucoidan-contg. extract is obtd. from the mozuku by bringing hydrogen peroxide into reaction with mozuku algar substances or adding the hydrogen peroxide to the mozuku extraction liquid. The mozuku algar substances are further subjected to a refining treatment after the hydrogen peroxide treatment to remove impurity of low mol.wt., by which the extract having a high fucoidan content is obtd. The mozuku extract of purity preferable as food is obtd. by removing the trace hydrogen peroxide remaining after the hydrogen peroxide treatment by a catalase treatment.
HIGH-MOLECULAR FUCOIDAN, METHOD OF PRODUCING THE SAME AND COSMETIC COMPOSITION
EP1854813
The present invention provides a method for producing high molecular-weight fucoidan, which is expected to be promising as a cosmetic composition or an agent in the field of dermatology, and a high molecular-weight fucoidan-containing composition. A component containing fucoidan having a weight average molecular weight of 1,000,000 to 2,000,000 obtained through the steps of performing hot water extraction of a mozuku alga body of the genus Cladosiphon at pH 6.0 or higher, which ranges from neutral to alkaline, removing low-molecular weight compound through ultrafiltration, and adjusting a final pH to 6.5 or higher is effective as a cosmetic composition or an agent in the field dermatology, and is also excellent in storage stability.
Process for preparing fucoidan by enzymatic hydrolysis of brown algae
CN1763212
The enzymically hydrolysis process of preparing fucan sulfate with brown algae includes the following steps: compounding composite cellulase with cellulase and pectase; enzymolyzing brown algae material via adding the composite water and cellulase at pH 6.0-8.0 for 50-80 min; heating at 90-100 deg.c in water bath for 3-4 hr, cooling to room temperature and centrifuging to obtain supernatant; adding alcohol to the supernatant to produce precipitate and centrifuging to obtain the second supernatant; adding alcohol to the second supernatant to produce precipitate and collecting the precipitate; washing the precipitate and vacuum freeze drying to obtain white powder. The said process has high extraction rate, low product cost, and less environmental pollution.
Method for extracting fucoidan and fucoxanthin from kelp
CN104448027
The invention relates to a method for extracting fucoidan and fucoxanthin from kelp. The method solves the technical problems that an existing method for extracting the fucoidan and the fucoxanthine from the kelp is long in extraction time, high in cost, serious in environment pollution and low in product yield. The method comprises the steps that the kelp is cleaned, smashed and put into an extraction tank together with a certain proportion of water, extraction is conducted at high temperature and under high pressure, hot filtration, cooling and sedimentation are conducted on an extraction solution, sediment is the fucoxanthin, and the fucoxanthin product is obtained after the drying process; then ultrafiltration concentration is conducted on supernatant liquid, filtrate is collected, trichloroacetic acid is added into the filtrate to remove protein, centrifugation is conducted, an ethanol solution is added into the supernatant liquid for fractional precipitation, sediment is collected, and the fucoidan is obtained after the vacuum drying process. The method can be used for extracting the fucoidan and the fucoxanthin from the kelp.
Method for preparing seaweed product for reducing blood pressure via step-by-step extraction and bioconversion coupling
CN104256252
The invention relates to a health care product for assisting blood pressure reduction, provides a method for preparing a health care seaweed product for assisting blood pressure reduction via step-by-step extraction and bioconversion coupling, and belongs to the field of deep processing of ocean foods. The method is carried out by taking high-quality seaweeds as raw materials via an ultrasonic circulation step-by-step extraction process and a bioconversion coupling preparation technology. The preparation method sequentially comprises the following steps: carrying out pretreatment of raw materials, alcohol extraction of mannitol, acid extraction and alcohol precipitation of sodium alginate and fucoidan, bioconversion and ultrafiltration so as to obtain physiological active components for reducing blood pressure; and scientifically blending the active components. According to the method, a large quantity of seafood seaweeds in China are deeply processed and comprehensively utilized so as to obtain five products, namely mannitol, sodium alginate, fucoidan, seaweed dietary fibers and seaweed antihypertensive peptides; and the five products are scientifically blended so as to develop a series of novel functional seaweed products for reducing blood pressure reduction, wherein the series of functional seaweed products have obvious functions of assisting blood pressure reduction. The ACE inhibitory activity IC50 of the seaweed product is within 0.68mg/g to 1.15mg/g, so that the blood pressure of an SHR (Spontaneously Hypertensive Rat) can be obviously lowered (-12.4 mmHg to -17.5mmHg).
Method for extracting fucus extract and application of fucus extract in anti-aging cosmetics
CN104173231
The invention aims to provide a method for extracting a fucus extract and application of the fucus extract in anti-aging cosmetics, namely providing fucoidan extracted from fucus by a mode of microwave assisted extraction and applying the fucoidan to cosmetics. The fucoidan extract has the effects of high safety, good oxidation resistance, senescence resistance, moisture retaining and moistening.
HIGH-YIELD, HIGH-PURITY FUCOIDAN MANUFACTURING METHODS
KR20140086445
The present invention relates to a method for manufacturing high-yield and high-purity fucoidan and, more specifically, to a method for manufacturing high-yield and high-purity fucoidan which enhances purity of extraction yield and fucoidan index materials (fucose, glucose, xylose, galactose, and sulfonic acid) by preventing loss of active ingredients in kelp by using a kelp. The method for manufacturing high-yield and high-purity fucoidan of the present invention comprises the following steps: heat plasticizing granule pulverized kelp using a roasting machine; extracting at low temperature using lyase; proliferating one or more strains selected among Lactobacillus brevis DL-25, Saccharomyces cerevisiae DS-7, and Aspergillus oryaze DF-11 for 10-30 hours in kelp extracts; then separating and purifying fermented culture fluid.
METHOD FOR EXTRACTING BROWN ALGAE POLYSACCHARIDE VIA MICROWAVE CHEMICAL PROCESS
US2014296496
This invention relates to an extraction process of brown algae polysaccharides in a field of pharmaceutical chemistry. This invention particularly discloses a process of extracting brown algae polysaccharides based on a microwave chemistry method and brown algae polysaccharides obtained by said process. The process of the invention comprises: 1) putting pulverized brown algae powder into a microwave reaction chamber, adding acid solution to conduct reaction; optionally concentrating the mixer, and then washing with organic solvent to remove excess acid; conducting grading alcohol precipitation after water extract to obtain mannuronic acid rich fragment (M rich) algin, fucoidan and/or laminaran respectively; and adding an alkali solution to the brown algae residue to conduct alkaline digestion, filtering the residue off, adjusting pH of the filtrate to neutral, conducting alcohol precipitation to obtain guluronic acid rich fragment (G rich) algin precipitates. The present invention has significant advantages like fast processing rate, high yield of polysaccharides, strong controllable polysaccharide degradation, using less organic acid and efficient recovery, small water consumption, low power consumption, etc., the active polysaccharides has high yield and content, better water-soluble, and good biological activities.
Method for extracting fucoidan polysaccharide sulfate
CN103980373
The invention relates to a novel extraction method of a plant crude drug, namely a method for extracting fucoidan polysaccharide sulfate. The name of the preparation commodity is a haikun shenxi capsule. The method comprises the following main extracting steps: (1) adding 10-fold water to a dried kelp to soak for 8 hours, controlling room temperature at 8-32 DEG C, taking a soak solution to be concentrated into 1/5 of original volume for later use, and separating out alginate on the surface of a marine organism by adopting the step; (2) taking out and cutting up the soaked kelp, and homogenating by using an AH high-pressure homogenizer; (3) adding sodium hydroxide to homogenated liquid to adjust the pH value to 12, stirring for 30 minutes, standing for 12 hours, adjusting the pH value to 1.5-2 by using a hydrochloric acid, stirring for 8 hours, and standing for 12 hours; (4) filtering; (5) merging filtrate with a concentrated solution in the step (1) and desalting by adopting specific 500 electroosmosis; (6) concentrating and decoloring soup; (7) centrifuging, and washing a filter cake by using 95% ethanol; (8) drying and crushing a dry substance. The yield is improved, and the cost is reduced. Compared with the prior art, the yield of the method is improved by over 40% by detection.
Method for extracting and preparing low molecular fucoidan from Ascophyllum mackaii
CN103788219
The invention relates to the alga chemical field, and concretely relates to a method for extracting and preparing low molecular fucoidan from Ascophyllum mackaii. The method is characterized in that Ascophyllum mackaii is immersed and extracted by employing dilute hydrochloric acid and water, a soak solution is used for purifying an extract through a foaming machine, an upper layer floater and a lower layer sediment are respectively collected, and polysaccharide in the Ascophyllum mackaii is fully extracted and collected by using a filtering collection and a cell disruption extraction method. An ultrafiltration technology is used for removing salinity and the graded products in 20000 Dalton can be obtained. The fucoidan crude product is obtained by an ethanol precipitation method. Purification and classification of an anion exchange resin chromatographic column of DEAE-Sepharose F.F. can be carried out. Next classification and concentration of an 8-14KD dialysis bag are carried out, and cryoconcentration and freeze drying are carried out to obtain the fucoidan product.
METHOD OF LOW MOLECULAR WEIGHT FUCOIDAN FROM BROWN SEAWEED LIMU MOUI THAT ORIGINATED FROM SOUTH PACIFIC OCEAN
KR101386006
The present invention relates to fucoidan extract and a method for lowering molecular weight of fucoidan using brown seaweed limu moui, and more specifically, as a technique of extracting, collecting fucoidan and lowering molecular weight of the fucoidan using natural brown seaweed limu moui in south pacific ocean, to fucoidan extract using limu moui and a manufacturing method of fucoidan which molecular weight is lowered, which wash, desalt, and triturate the brown algae limu moui, and then enzyme-treat, heat and react thereby lowering molecular weight of fucoidan and therefore the fucoidan can be absorbed well in human body. [Reference numerals] (AA) Limu moui; (BB) Cleaning, desalination; (CC) Tritaration; (DD) Enzyme treatment step; (EE) Reaction step; (FF) Extraction step; (GG) Separation step; (HH) Manufacture fucoidan
Method for coproducing fucoidan and seaweed fertilizer
CN103755831
The invention discloses a method for coproducing fucoidan and a seaweed fertilizer. The method comprises the following steps: (1) extracting a brown seaweed component having a plant growth promoting function; (2) extracting the fucoidan; (3) preparing a seaweed fertilizer component 2; and (4) blending the seaweed fertilizer, namely completely adding the seaweed fertilizer component 1 obtained in the step (1) into the seaweed fertilizer component 2 obtained in the step (3), stirring and mixing uniformly, standing for precipitating for 2 hours, filtering and centrifuging to perform liquid-solid separation, wherein the obtained liquid and solid refer to a liquid seaweed fertilizer base and a solid seaweed fertilizer base respectively. According to the method for coproducing the fucoidan and the seaweed fertilizer, resources are fully utilized, and the fucoidan product and the seaweed fertilizer are produced by means of the processes of ethanol soaking extraction, probiotics fermentation and the like, so that the problems of high water consumption as well as generation of waste water and waste residues in the production of the fucoidan are solved fundamentally, the additional value of the brown seaweed industry is increased remarkably, and a remarkable ecologic benefit is achieved.
Extraction device for kelp fucoidan
CN103665179
The invention provides an extraction device for kelp fucoidan. The extraction device comprises a crusher, an ultrasonic extraction tank, a first filter, a centrifuge, an extraction tank, a second filter and a freeze dryer which are sequentially connected. The extraction tank is connected with a dehydrating agent tank, the dehydrating agent tank is connected onto the extraction tank through a recycling tank, and a cooling device is arranged on the recycling tank. The extraction device for kelp fucoidan has the advantages that the extraction and purification step is simple, and the extraction efficiency is high; the adopted reagent is non-toxic and odorless, is biodegradable and cannot cause secondary pollution; the obtained fucoidan is safe in use and is suitable for industrial production.
A FUCOXANTHIN AND FUCOIDAN EXTRACTION METHOD FROM
KR20140002923
The present invention relates to a method for extracting fucoxanthin and fucoidan from seaweed. The method of the present invention comprises: a first step of pulverizing seaweed; a second step of wrapping the pulverized seaweed in a non-woven fabric; a third step of inserting ethanol into the seaweed to perform room-temperature extraction; a fourth step of extracting the extract from the third step at a temperature of 50 to 60°C; a fifth step of extracting fucoxanthin from a filtered solution in the extract from the fourth step; and a sixth step of extracting fucoidan from the residual seaweed in the extract from the fourth step. [Reference numerals] (A1) Fucoxanthin extracting·purifying; (A2) Fucoidan extracting·purifying; (BB) Pulverize dried seaweed; (CC) Wrap in a non-woven fabric; (D1) Insert ethanol; (D2) First room-temperature extraction (stirring or immersing); (EE,HH) Extract at 50°C-60°C; (FF,II,RR,TT) Filter; (G1) Put new seaweed into filtrate; (G2) Secondary room-temperature extraction (stirring or immersing); (JJ) Fucoxanthin extract; (KK) Ethanol vacuum-evaporation; (LL) Mix with excipient; (MM) Freeze-dry; (NN) Fucoxanthin powder; (OO) Residual seaweed; (PP) Insert water and organic acid; (QQ) First extracting at 90-100°C; (SS) Insert water, Second extracting at 100~110°C; (UU) Filtrate; (VV) Add CaCl_2; (WW) Remove alginic acid; (XX) Ultrafiltrating; (YY) Vacuum concentrating; (ZZ) Fucoidan liquid.
FUCOIDAN, AN ENZYME MANUFACTURING METHOD
KR20130121221
The present invention relates to fucoidan enzyme drink extracted from seaweeds using fermented liquid obtained by adding yeast culture liquid and lactic acid bacterial culture liquid to rice powder and fermenting the mixture to secure the flavor, taste, palatability, and preservation compare to an existing production method. The obtained fermented liquid promotes the fermentation by being used as an enzyme. Through a starter drink production process which obtains clear drink and acetic acid ferments the clear drink to extract fucoidan from the seaweeds, the seaweeds remove the smell of vinegar and alcohol and the vinegar containing weak acidic liquid removes various germs, and the salinity contained in original materials is removed. An extraction method of fucoidan obtains fucoidan enzyme liquid without alcohol by evaporating the alcohol through heating. The fucoidan which is contained in brown seaweeds is polysaccharide and is uneasy to be digested, but through the present invention including a fermentation process, the fucoidan is changed into low molecules so that the human body can easily absorb. [Reference numerals] (AA) Process of producing fermented liquid;(BB) Step of dipping;(CC) Aging step;(DD) Process of fermentated alcoholic beverage;(EE) Process of extracting fucoidan;(FF) Extracting step;(GG) Filtering step;(HH) Evaporating step;(II) Mixing step;(JJ) Red ginseng vinegar mixing step;(KK) Process of producing fucoidan enzyme;(LL) Finish.
Method for extracting fucoidan and removing heavy metals from kelps
CN103387622
The invention discloses a method for extracting fucoidan and removing heavy metals from kelps. The method comprises the following steps of: pre-treating raw materials; extracting the fucoidan; settling to obtain a crude product of the fucoidan; reversely extracting the fucoidan; removing the heavy metals; and secondarily settling to obtain the fucoidan. According to the invention, normal temperature ultrasonic extraction is adopted, so that the extracting efficiency is high, and the economic value of the kelps is increased; the cost and the energy consumption can be reduced, and the environment can not be polluted; and the obtained product is high in purity, low in impurity content, safe in eating and suitable for industrial production.
EXTRACTION METHOD OF FUCOIDAN
JP2013203737
PROBLEM TO BE SOLVED: To provide a method of producing a fucoidan extract by performing processes of cleaning, boiling with a citrus fruit extract, pressure sterilization, immersion in liquor, and filtration for extracting more fucoidan highly included in seaweed of the family Spermatochnaceae, kelp, Undaria pinnatifida, and other marine algae.SOLUTION: For increasing extraction efficiency of fucoidan highly included in seaweed of the family Spermatochnaceae, kelp, Undaria pinnatifida, and other marine algae, cleaning with fresh water and boiling with a citrus fruit extract are carried out to extract fucoidan, and when a filtration residue is immersed in liquor, a fucoidan extract further increased in its amount can be easily obtained at low cost.
MAKING METHOD OF FUCOIDAN FROM THE ROOT EXTRACT OF TANGLEWEED
KR20130084441
PURPOSE: A method for preparing physiologically active fucoidan using a tangleweed root extract is provided to prevent the loss of active ingredients in tangleweed roots through low temperature extraction and to improve extraction yield and the purity of a fucoidan marker. CONSTITUTION: A method for preparing physiologically active fucoidan using a tangleweed root extract comprises the steps of: removing foreign materials from dried tangleweed roots (S100); crushing the dried tangleweed roots (S200); putting the dried tangleweed roots into a roasting machine and spraying with alcohol; rotating at low speed; closing the roasting machine when the fragrance of the tangleweed roots is vaporized; adding flavor to the tangleweed roots and plasticizing (S300); washing the plasticized tangleweed roots (S400); adding distilled water to the tangleweed roots, adding lyase, and extracting the tangleweed roots at 35-50 °C (S500); adding distilled water to the tangleweed roots and extracting in a vacuum condition at 35-50 °C (S600); putting the extract into a fermentor and culturing one or more kinds of strains in the extract for 10-30 hours (S700); and isolating and purifying the fermented culture liquid and distilling (S800). [Reference numerals] (AA) Start; (BB) First process; (CC) Second process; (DD) Third process; (EE) End; (S100) Wash; (S200) Crush granules; (S300) Thermal plasticizing; (S400) Wash with distilled water; (S500) Enzymatic lysis/extraction at low temperature; (S600) Extract under a vacuum condition at low temperature; (S700) Fermenting with microorganism; (S800) Isolation/purification/distillation/concentration.
EXTRACTING METHOD OF HIGHLY PURIFIED FUCOIDAN
KR20130055926
PURPOSE: A high purity fucoidan extraction method is provided to develop various application techniques using processed by-products. CONSTITUTION: A high purityfucoidan extraction method comprises: a step of drying and pulverizing seaweed-processed by-products; a step of decomposing and extracting the by-products using an enzyme to obtain an extract; a step of preparing fractions of each molecular weight by an ultrafiltration; a step of adding spirit to each fraction; and a step of collecting and freeze-drying precipitated polysaccharides. The seaweed is Hizikia fusiformis(Harvey) Okamura or Ecklonia cava.
Method for preparing fucoidan
CN101993501
The invention provides a method for preparing fucoidan, relating to the field of production and application of food additives. The method comprises the following steps of: adding distilled water according to a material to liquid ratio of 1:40-4:80 g/mL with brown algae, such as kelps, undaria pinnatifida, and the like as raw materials; treating by a colloid mill and then carrying out microwave-assisted extraction or ultrasonic wave-assisted extraction, wherein, in the microwave-assisted extraction process, the microwave time lasts 20-40min, the microwave extraction temperature is 60-80 DEG C, and the microwave power is 400-500W; and in the ultrasonic wave-assisted extraction process, the ultrasonic power is 800-100W, the microwave time lasts 20-40min, and the extraction temperature is 60-90 DEG C; and centrifuging an extracting solution, adding a proper amount of 2% CaCl2 solution into a supernatant, stirring, keeping constant temperature for 20-30h at 35-40 DEG C, centrifuging, adding 50-100% ethanol into the supernatant until the ethanol concentration of the solution is 15-20%, stirring, filtering, concentrating, adding 90-100% ethanol into a concentrated solution until the ethanol concentration of the solution is 55-60%, centrifuging again and drying a precipitate in vacuum to obtain white or yellow-white fucoidan powder.